#ifndef CODE_Transcript #define CODE_Transcript #include "IncludeDefine.h" #include "Parameters.h" #include "Variation.h" #include "Genome.h" #include class Transcript { public: uint exons[MAX_N_EXONS][EX_SIZE]; //coordinates of all exons: r-start, g-start, length vector > cigar; //new way to record alignments, with CIGAR operations. For now, only used by splice-graph uint shiftSJ[MAX_N_EXONS][2]; //shift of the SJ coordinates due to genomic micro-repeats int canonSJ[MAX_N_EXONS]; //canonicity of each junction uint8 sjAnnot[MAX_N_EXONS]; //anotated or not uint8 sjStr[MAX_N_EXONS]; //strand of the junction uint intronMotifs[3]; uint8 sjMotifStrand; bool sjYes; uint nExons; //number of exons in the read transcript //variables from ReadAlign uint *readLengthOriginal, *readLength; uint Lread, readLengthPairOriginal; uint iRead; //read identifier uint readNmates; char *readName; int iFrag; //frag number of the transcript, if the the transcript contains only one frag //loci uint rStart, roStart, rLength, gStart, gLength, cStart; //read, original read, and genomic start/length, chromosome start uint Chr,Str,roStr; //chromosome and strand and original read Strand uint32 haploType; //haplotype index for diploid genome bool primaryFlag; uint nMatch;//min number of matches uint nMM;//max number of mismatches uint mappedLength; //total mapped length, sum of lengths of all blocks(exons) uint extendL; //extension length intScore maxScore; //maximum Score uint nGap, lGap; //number of genomic gaps (>alignIntronMin) and their total length uint nDel; //number of genomic deletions (ie genomic gaps) uint nIns; //number of (ie read gaps) uint lDel; //total genomic deletion length uint lIns; //total genomic insertion length uint nUnique, nAnchor; //number of unique pieces in the alignment, number of anchor pieces in the alignment vector varInd; vector varGenCoord, varReadCoord ; vector varAllele; //annotations std::set alignGenes; Transcript(); //resets to 0 void reset(); //reset to 0 void resetMapG(); // reset map to 0 void resetMapG(uint); // reset map to 0 for Lread bases void add(Transcript*); // add intScore alignScore(char **Read1, char *G, Parameters &P); int variationAdjust(const Genome &mapGen, char *R); string generateCigarP(); //generates CIGAR void peOverlapSEtoPE(uint* mSta, const Transcript &t); void extractSpliceJunctions(vector> &sjOut, bool &annotYes); uint64 chrStartLengthExtended(); bool transformGenome(Genome &genOut, Transcript & A); bool convertGenomeCigar(Genome &genOut, Transcript & A); private: }; #endif