1<multi-test> 2 <plugin_primer_3> 3 <plugin_primer_3_in 4 5 PRIMER_SEQUENCE_ID="mispriming" 6 PRIMER_PICK_INTERNAL_OLIGO="1" 7 PRIMER_SELF_ANY="11" 8 PRIMER_MAX_POLY_X="100" 9 PRIMER_INTERNAL_OLIGO_MAX_POLY_X="100" 10 PRIMER_SELF_END="12" 11 PRIMER_NUM_RETURN="1" 12 SEQUENCE="TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG" 13 TARGET="21,122" 14 PRIMER_LEFT_INPUT="CCTACAGCTGTGGGAAAATG" 15 PRIMER_INTERNAL_OLIGO_INPUT="gcgtacgatccatgctagc" 16 PRIMER_INTERNAL_OLIGO_MIN_TM="60" 17 PRIMER_MAX_TM="100" 18 PRIMER_MAX_GC="100" 19 PRIMER_MISPRIMING_LIBRARY="not_for_production.lib" 20 PRIMER_MAX_MISPRIMING="8" 21 PRIMER_EXPLAIN_FLAG="1" 22 PRIMER_PICK_ANYWAY="1" 23 24 /> 25 <plugin_primer_3_out 26 PRIMER_PAIRS_NUMBER="1" 27 PRIMER_PAIR_PENALTY="4.0864" 28 PRIMER_LEFT_PENALTY="1.771733" 29 PRIMER_RIGHT_PENALTY="2.314641" 30 PRIMER_INTERNAL_OLIGO_PENALTY="1.580164" 31 PRIMER_LEFT_SEQUENCE="CCTACAGCTGTGGGAAAATG" 32 PRIMER_RIGHT_SEQUENCE="CAGTGTGTACATGGCGGC" 33 PRIMER_INTERNAL_OLIGO_SEQUENCE="gcgtacgatccatgctagc" 34 PRIMER_LEFT="1,20" 35 PRIMER_RIGHT="160,18" 36 PRIMER_INTERNAL_OLIGO="23,19" 37 PRIMER_LEFT_TM="58.228" 38 PRIMER_RIGHT_TM="59.685" 39 PRIMER_INTERNAL_OLIGO_TM="59.420" 40 PRIMER_LEFT_GC_PERCENT="50.000" 41 PRIMER_RIGHT_GC_PERCENT="61.111" 42 PRIMER_INTERNAL_OLIGO_GC_PERCENT="57.895" 43 PRIMER_LEFT_SELF_ANY="10.00" 44 PRIMER_RIGHT_SELF_ANY="6.00" 45 PRIMER_INTERNAL_OLIGO_SELF_ANY="6.00" 46 PRIMER_LEFT_SELF_END="0.00" 47 PRIMER_RIGHT_SELF_END="3.00" 48 PRIMER_INTERNAL_OLIGO_SELF_END="6.00" 49 PRIMER_LEFT_MISPRIMING_SCORE="9.00, L1 Human L1 interspersed repetitive sequence - full length copy" 50 PRIMER_RIGHT_MISPRIMING_SCORE="8.00, L1 Human L1 interspersed repetitive sequence - full length copy" 51 PRIMER_PAIR_MISPRIMING_SCORE="17.00, L1 Human L1 interspersed repetitive sequence - full length copy" 52 PRIMER_LEFT_END_STABILITY="7.2000" 53 PRIMER_RIGHT_END_STABILITY="12.9000" 54 PRIMER_PAIR_COMPL_ANY="4.00" 55 PRIMER_PAIR_COMPL_END="1.00" 56 PRIMER_PRODUCT_SIZE="160" 57 /> 58 </plugin_primer_3> 59</multi-test> 60