sequence/unit_test/polya.seq.asn

Seq-entry ::= seq {
  id {
    general {
      db "dbEST",
      tag id 1216776
    },
    genbank {
      accession "AA566393",
      version 1
    },
    gi 2339025
  },
  descr {
    molinfo {
      biomol mRNA,
      tech est
    },
    title "ZF-E478 zebrafish embryonic Danio rerio cDNA clone ZF-E478 3' end.",
    create-date std {
      year 1997,
      month 8,
      day 20
    },
    update-date std {
      year 1998,
      month 8,
      day 25
    },
    source {
      org {
        taxname "Danio rerio",
        common "zebrafish",
        db {
          {
            db "taxon",
            tag id 7955
          }
        },
        orgname {
          name binomial {
            genus "Danio",
            species "rerio"
          },
          mod {
            {
              subtype other,
              subname "Vector: Lambda Uni-ZAP XR; Site_1: EcoRI; Site_2: XhoI;
 mRNA was purified from mixed stages of zebrafish embryos from 6 hours
 postfertilization to hatching.  cDNA was unidirectionally cloned into the
 lambda ZAPII vector using Stratagene's Lambda Uni-ZAP XR cDNA cloning system"
            }
          },
          lineage "Eukaryota; Metazoa; Chordata; Craniata; Vertebrata;
 Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
 Cypriniformes; Cyprinidae; Danio",
          gcode 1,
          mgcode 2,
          div "VRT"
        }
      },
      subtype {
        {
          subtype clone,
          name "ZF-E478"
        },
        {
          subtype clone-lib,
          name "LIBEST_000356 zebrafish embryonic"
        }
      }
    }
  },
  inst {
    repr raw,
    mol rna,
    length 197,
    strand ss,
    seq-data ncbi2na 'FFFFFFFFFBFF1FFA000BF3EE0387BB803EA3AA0CFA497A512C1B4EE5
FC1112708AE7B6015E86A68800A02415D1F4E2E97FC0'H
  },
  annot {
    {
      desc {
        name "NCBI_GPIPE"
      },
      data ftable {
        {
          data region "alignable",
          location int {
            from 24,
            to 196,
            strand minus,
            id gi 2339025
          }
        },
        {
          data region "polya",
          location int {
            from 0,
            to 23,
            strand minus,
            id gi 2339025
          },
          qual {
            {
              qual "method",
              val "FindPolyTail"
            }
          }
        }
      }
    }
  }
}
Seq-entry ::= seq {
  id {
    general {
      db "dbEST",
      tag id 1216909
    },
    genbank {
      accession "AA566526",
      version 1
    },
    gi 2339158
  },
  descr {
    molinfo {
      biomol mRNA,
      tech est
    },
    title "ZF-ES47 zebrafish embryonic (subtraction) Danio rerio cDNA clone
 ZF-ES47 5' end.",
    create-date std {
      year 1997,
      month 8,
      day 20
    },
    update-date std {
      year 1998,
      month 8,
      day 25
    },
    source {
      org {
        taxname "Danio rerio",
        common "zebrafish",
        db {
          {
            db "taxon",
            tag id 7955
          }
        },
        orgname {
          name binomial {
            genus "Danio",
            species "rerio"
          },
          mod {
            {
              subtype other,
              subname "Organ: embryo; Vector: Lambda Uni-ZAP XR; Site_1:
 EcoRI; Site_2: XhoI; mRNA was purified from mixed stages of zebrafish embryos
 from 6  hours postfertilization to hatching.  cDNA was unidirectionally
 cloned  into the lambda ZAPII vector using the Stratagene's Lambda Uni-ZAP XR
  cDNA cloning system. "
            }
          },
          lineage "Eukaryota; Metazoa; Chordata; Craniata; Vertebrata;
 Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
 Cypriniformes; Cyprinidae; Danio",
          gcode 1,
          mgcode 2,
          div "VRT"
        }
      },
      subtype {
        {
          subtype clone,
          name "ZF-ES47"
        },
        {
          subtype clone-lib,
          name "LIBEST_000970 zebrafish embryonic (subtraction)"
        },
        {
          subtype tissue-type,
          name "embryonic"
        },
        {
          subtype dev-stage,
          name "mixed from 6 hours postfertilization to hatching"
        }
      }
    }
  },
  inst {
    repr raw,
    mol rna,
    length 220,
    strand ss,
    seq-data ncbi2na 'B512AD40C021E0E0D388050A1010388234EA3E222D2EEF087FFBFEEA
2B34FD37BF3BF4E03F38D0F0A9FFFFBFEC50C021EFC78000000000'H
  },
  annot {
    {
      desc {
        name "NCBI_GPIPE"
      },
      data ftable {
        {
          data region "alignable",
          location int {
            from 0,
            to 200,
            strand plus,
            id gi 2339158
          }
        },
        {
          data region "polya",
          location int {
            from 201,
            to 219,
            strand plus,
            id gi 2339158
          },
          qual {
            {
              qual "method",
              val "FindPolyTail"
            }
          }
        }
      }
    }
  }
}
Seq-entry ::= seq {
  id {
    general {
      db "dbEST",
      tag id 1188667
    },
    genbank {
      accession "AA542596",
      version 1
    },
    gi 2289531
  },
  descr {
    molinfo {
      biomol mRNA,
      tech est
    },
    title "fa08e08.s1 Zebrafish ICRFzfls Danio rerio cDNA clone 5A5 3'.",
    create-date std {
      year 1997,
      month 7,
      day 31
    },
    update-date std {
      year 1997,
      month 7,
      day 31
    },
    source {
      org {
        taxname "Danio rerio",
        common "zebrafish",
        db {
          {
            db "taxon",
            tag id 7955
          }
        },
        orgname {
          name binomial {
            genus "Danio",
            species "rerio"
          },
          mod {
            {
              subtype other,
              subname "Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1st strand
 cDNA was primed with a Not I - oligo(dT)15 primer [5
'pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT3'], on mRNA from pooled 26
 somite zebrafish embryos; double-stranded cDNA was ligated to Sal I adaptors
 (BRL), digested with Not I and cloned into the Not I and Sal I sites of the
 pSPORT1 vector (BRL). Library was constructed by Matthew Clark (Lehrach lab;
 ICRF, London and  Max Planck Institut fuer Molekulare Genetik, Berlin) and
 was not biochemically normalised.  70,000 clones from this library were
 arrayed on high density filters and subsequently screened by oligonucleotide
 hybridization fingerprinting to identify unique or minimally redundant clones
 for more intensive analysis."
            }
          },
          lineage "Eukaryota; Metazoa; Chordata; Craniata; Vertebrata;
 Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
 Cypriniformes; Cyprinidae; Danio",
          gcode 1,
          mgcode 2,
          div "VRT"
        }
      },
      subtype {
        {
          subtype clone,
          name "5A5"
        },
        {
          subtype clone-lib,
          name "LIBEST_000899 Zebrafish ICRFzfls"
        },
        {
          subtype sex,
          name "mixed"
        },
        {
          subtype tissue-type,
          name "pooled 26-somite embryos"
        },
        {
          subtype lab-host,
          name "XL1-blue MRF"
        }
      }
    }
  },
  inst {
    repr raw,
    mol rna,
    length 418,
    strand ss,
    seq-data ncbi2na 'FFFFFFF03504CFF3FE2013F08CFFA24B013B4A7030F484CD3E1F79ED
F4F2FD001103B7F10FC03A41FE8CEFF79E8AC7B2FA4BFCC17E0FF501EEB317E10EBCD954E5CC44
B31EC01504B43FCD03C0EECBC1D00FCF80BC3CC741E008BFC0B43BE0AC38AC3C0C5D3C7D07C0'H
  },
  annot {
    {
      desc {
        name "NCBI_GPIPE"
      },
      data ftable {
        {
          data region "alignable",
          location int {
            from 14,
            to 371,
            strand minus,
            id gi 2289531
          }
        },
        {
          data region "polya",
          location int {
            from 0,
            to 13,
            strand minus,
            id gi 2289531
          },
          qual {
            {
              qual "method",
              val "FindPolyTail"
            }
          }
        }
      }
    }
  }
}
Seq-entry ::= seq {
  id {
    general {
      db "dbEST",
      tag id 24185128
    },
    genbank {
      accession "CO354973",
      version 1
    },
    gi 49436290
  },
  descr {
    molinfo {
      biomol mRNA,
      tech est
    },
    title "DR_ATE_NRM03_A07 adult testis normalized (TLL) Danio rerio cDNA.",
    create-date std {
      year 2004,
      month 6,
      day 29
    },
    update-date std {
      year 2004,
      month 6,
      day 29
    },
    source {
      org {
        taxname "Danio rerio",
        common "zebrafish",
        db {
          {
            db "taxon",
            tag id 7955
          }
        },
        orgname {
          name binomial {
            genus "Danio",
            species "rerio"
          },
          mod {
            {
              subtype strain,
              subname "AB"
            },
            {
              subtype other,
              subname "Organ: pooled testis; Vector: pBluescript II SK;
 Site_1: EcoRI; Site_2: XhoI; Full length cDNA was synthesized from adult
 testis poly(A)+ RNA with ZAP-cDNA Synthesis Kit (Stratagene).
 Size-fractionated cDNA (mainly 0.5-3kb in length) were directionally cloned
 into Uni-ZAP XR vector. The cDNA clones were excised from Uni-ZAP vector
 using ExAssist helper phage. Excised pBluescript phagemids were used to
 transform E coli XL1-Blue cells. The plasmid library was normalized by using
 a reassociation-kinetics-based approach according to Bonaldo et al., (Genome
 Research 6: 791-806,1996) with minor modifications. The insert from randomly
 selected white colonies was PCR amplified using M13 forward and reverse
 primers and partially sequenced by using M13 reverse primer."
            }
          },
          lineage "Eukaryota; Metazoa; Chordata; Craniata; Vertebrata;
 Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
 Cypriniformes; Cyprinidae; Danio",
          gcode 1,
          mgcode 2,
          div "VRT"
        }
      },
      subtype {
        {
          subtype clone-lib,
          name "LIBEST_015931 adult testis normalized (TLL)"
        },
        {
          subtype sex,
          name "male"
        },
        {
          subtype dev-stage,
          name "adult (fully mature)"
        }
      }
    }
  },
  inst {
    repr raw,
    mol rna,
    length 500,
    strand ss,
    seq-data ncbi2na '70453E20E13E77AD09E1E4525DDE0E74EED57BF97A78A7D2307F977A
4E17BB87DE8EABB852310E77BD1578E27524AD9DF51EFB50E08F908147AA0904D6A114E3F992B9
E7AE5D75E4EAE1DEBAD77ADED20A30CDE877AEACFBB5EA924B6FB8571CE5E9BB1A58B4589D6E1E
AE8D23DE97507030300EC49437FA4D1E000000'H
  },
  annot {
    {
      desc {
        name "NCBI_GPIPE"
      },
      data ftable {
        {
          data region "alignable",
          location int {
            from 0,
            to 487,
            strand plus,
            id gi 49436290
          }
        },
        {
          data region "polya",
          location int {
            from 488,
            to 499,
            strand plus,
            id gi 49436290
          },
          qual {
            {
              qual "method",
              val "FindPolyTail"
            }
          }
        }
      }
    }
  }
}
Seq-entry ::= seq {
  id {
    general {
      db "dbEST",
      tag id 41387444
    },
    embl {
      accession "CT700515",
      version 2
    },
    gi 112736981
  },
  descr {
    molinfo {
      biomol mRNA,
      tech est
    },
    title "CT700515 ZF_mu Danio rerio cDNA clone ZF_mu_199p09 3'.",
    create-date std {
      year 2006,
      month 3,
      day 16
    },
    update-date std {
      year 2006,
      month 8,
      day 29
    },
    source {
      org {
        taxname "Danio rerio",
        common "zebrafish",
        db {
          {
            db "taxon",
            tag id 7955
          }
        },
        orgname {
          name binomial {
            genus "Danio",
            species "rerio"
          },
          mod {
            {
              subtype other,
              subname "Vector: pSPORT1; Site_1: SalI; Site_2: NotI; SalI_NotI
 cut cDNA was then ligated into pSPORT1 with SalI at the 5' end and NotI at
 the 3' end."
            }
          },
          lineage "Eukaryota; Metazoa; Chordata; Craniata; Vertebrata;
 Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
 Cypriniformes; Cyprinidae; Danio",
          gcode 1,
          mgcode 2,
          div "VRT"
        }
      },
      subtype {
        {
          subtype clone,
          name "ZF_mu_199p09"
        },
        {
          subtype clone-lib,
          name "LIBEST_019253 ZF_mu"
        },
        {
          subtype dev-stage,
          name "myoblast"
        },
        {
          subtype lab-host,
          name "E. coli"
        }
      }
    }
  },
  inst {
    repr raw,
    mol rna,
    length 626,
    strand ss,
    seq-data ncbi2na 'FFFFFFFFFFFFFFFF15F7E53E6A4D52DFCFCFBC05088355033D375774
4030BB101E0BAAE4B81F4048DF4D6AEF7D2DE1DFFF24FE181E3BB85532315121D9EA7DD34CB5B5
2DFDDF92BE3F4A81EAE86FDE559C924014978AF80361EBD4A5D8474079147B7C05FD1EDF79712A
033111CBB878B820C8B1107B033D3110F93DF47EB13EEEFFBC0AAEB522ECFEC2BFAFBBC0003902
438B9F30F0FEF800D17CFFF0'H,
    hist {
      replaces {
        date std {
          year 2006,
          month 3,
          day 16
        },
        ids {
          gi 89911033
        }
      }
    }
  },
  annot {
    {
      desc {
        name "NCBI_GPIPE"
      },
      data ftable {
        {
          data region "alignable",
          location int {
            from 32,
            to 625,
            strand minus,
            id gi 112736981
          }
        },
        {
          data region "polya",
          location int {
            from 0,
            to 31,
            strand minus,
            id gi 112736981
          },
          qual {
            {
              qual "method",
              val "FindPolyTail"
            }
          }
        }
      }
    }
  }
}
Seq-entry ::= seq {
  id {
    general {
      db "dbEST",
      tag id 41534061
    },
    embl {
      accession "CT625722",
      version 2
    },
    gi 113380667
  },
  descr {
    molinfo {
      biomol mRNA,
      tech est
    },
    title "CT625722 ZF_mu Danio rerio cDNA clone ZF_mu_74p19 3'.",
    create-date std {
      year 2006,
      month 3,
      day 16
    },
    update-date std {
      year 2006,
      month 8,
      day 29
    },
    source {
      org {
        taxname "Danio rerio",
        common "zebrafish",
        db {
          {
            db "taxon",
            tag id 7955
          }
        },
        orgname {
          name binomial {
            genus "Danio",
            species "rerio"
          },
          mod {
            {
              subtype other,
              subname "Vector: pSPORT1; Site_1: SalI; Site_2: NotI; SalI_NotI
 cut cDNA was then ligated into pSPORT1 with SalI at the 5' end and NotI at
 the 3' end."
            }
          },
          lineage "Eukaryota; Metazoa; Chordata; Craniata; Vertebrata;
 Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
 Cypriniformes; Cyprinidae; Danio",
          gcode 1,
          mgcode 2,
          div "VRT"
        }
      },
      subtype {
        {
          subtype clone,
          name "ZF_mu_74p19"
        },
        {
          subtype clone-lib,
          name "LIBEST_019253 ZF_mu"
        },
        {
          subtype dev-stage,
          name "myoblast"
        },
        {
          subtype lab-host,
          name "E. coli"
        }
      }
    }
  },
  inst {
    repr raw,
    mol rna,
    length 392,
    strand ss,
    seq-data ncbi2na 'FFFFFFEC73BF3F7E8F3F0B03003EC3F7FC0C3BEDCA27B3FC30FF1C13
32901333B153F3B9133B13300CA006943FCC4ED13324005CCD005CCF0040EFCCCAF7F53BA9BFD5
553511E11A3FC83009A5DF493FC00E75BFD8AF60077A6CBFA07386C0EC9006'H,
    hist {
      replaces {
        date std {
          year 2006,
          month 3,
          day 16
        },
        ids {
          gi 89915898
        }
      }
    }
  },
  annot {
    {
      desc {
        name "NCBI_GPIPE"
      },
      data ftable {
        {
          data region "alignable",
          location int {
            from 11,
            to 391,
            strand minus,
            id gi 113380667
          }
        },
        {
          data region "polya",
          location int {
            from 0,
            to 10,
            strand minus,
            id gi 113380667
          },
          qual {
            {
              qual "method",
              val "FindPolyTail"
            }
          }
        }
      }
    }
  }
}
Seq-entry ::= seq {
  id {
    general {
      db "dbEST",
      tag id 1139420
    },
    genbank {
      accession "AA494743",
      version 1
    },
    gi 2225171
  },
  descr {
    molinfo {
      biomol mRNA,
      tech est
    },
    title "fa11h12.s1 Zebrafish ICRFzfls Danio rerio cDNA clone 14N22 3'
 similar to SW:CS24_HUMAN P23246 MYOBLAST CELL SURFACE ANTIGEN 24.1D5 ;.",
    create-date std {
      year 1997,
      month 6,
      day 27
    },
    update-date std {
      year 1997,
      month 6,
      day 27
    },
    source {
      org {
        taxname "Danio rerio",
        common "zebrafish",
        db {
          {
            db "taxon",
            tag id 7955
          }
        },
        orgname {
          name binomial {
            genus "Danio",
            species "rerio"
          },
          mod {
            {
              subtype other,
              subname "Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1st strand
 cDNA was primed with a Not I - oligo(dT)15 primer [5
'pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT3'], on mRNA from pooled 26
 somite zebrafish embryos; double-stranded cDNA was ligated to Sal I adaptors
 (BRL), digested with Not I and cloned into the Not I and Sal I sites of the
 pSPORT1 vector (BRL). Library was constructed by Matthew Clark (Lehrach lab;
 ICRF, London and  Max Planck Institut fuer Molekulare Genetik, Berlin) and
 was not biochemically normalised.  70,000 clones from this library were
 arrayed on high density filters and subsequently screened by oligonucleotide
 hybridization fingerprinting to identify unique or minimally redundant clones
 for more intensive analysis."
            }
          },
          lineage "Eukaryota; Metazoa; Chordata; Craniata; Vertebrata;
 Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
 Cypriniformes; Cyprinidae; Danio",
          gcode 1,
          mgcode 2,
          div "VRT"
        }
      },
      subtype {
        {
          subtype clone,
          name "14N22"
        },
        {
          subtype clone-lib,
          name "LIBEST_000899 Zebrafish ICRFzfls"
        },
        {
          subtype sex,
          name "mixed"
        },
        {
          subtype tissue-type,
          name "pooled 26-somite embryos"
        },
        {
          subtype lab-host,
          name "XL1-blue MRF"
        }
      }
    }
  },
  inst {
    repr raw,
    mol rna,
    length 540,
    strand ss,
    seq-data ncbi2na 'FFFFFFFAAFEA2050791DFCF803B1382CFF03B83A4481125320234810
3F10CF452CC110904E208C0872C62CA8F13BC824739FBDE1F4BEFF3CF0173C20C40300338E0000
1F9440EA38BFD29C01E4200F7F01F031343EE1DC0D22DDE83D44C427F9FF02E5234826E9F7E92B
5340F5D1A15E5035E0FD49143957785578945F9E80634F993B6FA19CA5'H
  },
  annot {
    {
      desc {
        name "NCBI_GPIPE"
      },
      data ftable {
        {
          data region "alignable",
          location int {
            from 14,
            to 338,
            strand minus,
            id gi 2225171
          }
        },
        {
          data region "polya",
          location int {
            from 0,
            to 13,
            strand minus,
            id gi 2225171
          },
          qual {
            {
              qual "method",
              val "FindPolyTail"
            }
          }
        }
      }
    }
  }
}
Seq-entry ::= seq {
  id {
    general {
      db "dbEST",
      tag id 2374564
    },
    genbank {
      accession "AI558400",
      version 1
    },
    gi 4508638
  },
  descr {
    molinfo {
      biomol mRNA,
      tech est
    },
    title "fb72f05.y1 Zebrafish WashU MPIMG EST Danio rerio cDNA clone
 IMAGE:3717441 5'.",
    create-date std {
      year 1999,
      month 3,
      day 24
    },
    update-date std {
      year 2001,
      month 6,
      day 7
    },
    source {
      org {
        taxname "Danio rerio",
        common "zebrafish",
        db {
          {
            db "taxon",
            tag id 7955
          }
        },
        orgname {
          name binomial {
            genus "Danio",
            species "rerio"
          },
          mod {
            {
              subtype other,
              subname "Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1st strand
 cDNA was primed with a Not I - oligo(dT)15 primer [5
'pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT3']; double-stranded cDNA was
 ligated to Sal I adaptors (BRL), digested with Not I and cloned into the Not
 I and Sal I sites of the pSPORT1 vector (BRL). Library was constructed by
 Matthew Clark (Lehrach lab; ICRF, London and Max Planck Institut fuer
 Molekulare Genetik,Berlin). cDNAs for EST analysis were selected following
 oligonucleotide hybridization fingerprinting of arrayed clones from zebrafish
 late somitogenesis (26 ss), adult liver or embryonic shield stage (5.6 h)
 libraries.  Fingerprint data were used to computationally cluster cDNAs, and
 a single cDNA from each cluster was chosen for sequencing.  In some cases
 multiple members of the same cluster were sequenced to assess clustering
 parameters or single clones were sequenced additional times to assess quality
 control."
            }
          },
          lineage "Eukaryota; Metazoa; Chordata; Craniata; Vertebrata;
 Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
 Cypriniformes; Cyprinidae; Danio",
          gcode 1,
          mgcode 2,
          div "VRT"
        }
      },
      subtype {
        {
          subtype clone,
          name "IMAGE:3717441"
        },
        {
          subtype clone-lib,
          name "LIBEST_001523 Zebrafish WashU MPIMG EST"
        },
        {
          subtype sex,
          name "mixed"
        },
        {
          subtype tissue-type,
          name "26 somite embryos, adult livers, shield stage embryos"
        },
        {
          subtype lab-host,
          name "XL1-blue MRF"
        }
      }
    }
  },
  inst {
    repr raw,
    mol rna,
    length 297,
    strand ss,
    seq-data ncbi2na '60A37BF874AA60A25E6BDA99E3A3A7BBA927BBA38137037BE54B3846
48782849781DCC5D2E02015F7EE876E2FE76FBA6FF860E9B9E3F77B533EFEE9FFB1210C0C3B8E0
1400000000000000'H
  },
  annot {
    {
      desc {
        name "NCBI_GPIPE"
      },
      data ftable {
        {
          data region "alignable",
          location int {
            from 0,
            to 270,
            strand plus,
            id gi 4508638
          }
        },
        {
          data region "polya",
          location int {
            from 271,
            to 296,
            strand plus,
            id gi 4508638
          },
          qual {
            {
              qual "method",
              val "FindPolyTail"
            }
          }
        }
      }
    }
  }
}
Seq-entry ::= seq {
  id {
    general {
      db "dbEST",
      tag id 1139245
    },
    genbank {
      accession "AA494568",
      version 1
    },
    gi 2224996
  },
  descr {
    molinfo {
      biomol mRNA,
      tech est
    },
    title "fa10b03.s1 Zebrafish ICRFzfls Danio rerio cDNA clone 8C6 3'.",
    create-date std {
      year 1997,
      month 6,
      day 27
    },
    update-date std {
      year 1997,
      month 6,
      day 27
    },
    source {
      org {
        taxname "Danio rerio",
        common "zebrafish",
        db {
          {
            db "taxon",
            tag id 7955
          }
        },
        orgname {
          name binomial {
            genus "Danio",
            species "rerio"
          },
          mod {
            {
              subtype other,
              subname "Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1st strand
 cDNA was primed with a Not I - oligo(dT)15 primer [5
'pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT3'], on mRNA from pooled 26
 somite zebrafish embryos; double-stranded cDNA was ligated to Sal I adaptors
 (BRL), digested with Not I and cloned into the Not I and Sal I sites of the
 pSPORT1 vector (BRL). Library was constructed by Matthew Clark (Lehrach lab;
 ICRF, London and  Max Planck Institut fuer Molekulare Genetik, Berlin) and
 was not biochemically normalised.  70,000 clones from this library were
 arrayed on high density filters and subsequently screened by oligonucleotide
 hybridization fingerprinting to identify unique or minimally redundant clones
 for more intensive analysis."
            }
          },
          lineage "Eukaryota; Metazoa; Chordata; Craniata; Vertebrata;
 Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
 Cypriniformes; Cyprinidae; Danio",
          gcode 1,
          mgcode 2,
          div "VRT"
        }
      },
      subtype {
        {
          subtype clone,
          name "8C6"
        },
        {
          subtype clone-lib,
          name "LIBEST_000899 Zebrafish ICRFzfls"
        },
        {
          subtype sex,
          name "mixed"
        },
        {
          subtype tissue-type,
          name "pooled 26-somite embryos"
        },
        {
          subtype lab-host,
          name "XL1-blue MRF"
        }
      }
    }
  },
  inst {
    repr raw,
    mol rna,
    length 170,
    strand ss,
    seq-data ncbi2na 'FFFEF200B9029EFC3830E0444F70F0B3CC3100C053923910C276CF42
753C07B21EE9EF2221D00EF48E9800'H
  },
  annot {
    {
      desc {
        name "NCBI_GPIPE"
      },
      data ftable {
        {
          data region "alignable",
          location int {
            from 7,
            to 158,
            strand minus,
            id gi 2224996
          }
        },
        {
          data region "polya",
          location int {
            from 0,
            to 6,
            strand minus,
            id gi 2224996
          },
          qual {
            {
              qual "method",
              val "FindPolyTail"
            }
          }
        }
      }
    }
  }
}
Seq-entry ::= seq {
  id {
    general {
      db "dbEST",
      tag id 1139254
    },
    genbank {
      accession "AA494577",
      version 1
    },
    gi 2225005
  },
  descr {
    molinfo {
      biomol mRNA,
      tech est
    },
    title "fa10c06.s1 Zebrafish ICRFzfls Danio rerio cDNA clone 23C8 3'.",
    create-date std {
      year 1997,
      month 6,
      day 27
    },
    update-date std {
      year 1997,
      month 6,
      day 27
    },
    source {
      org {
        taxname "Danio rerio",
        common "zebrafish",
        db {
          {
            db "taxon",
            tag id 7955
          }
        },
        orgname {
          name binomial {
            genus "Danio",
            species "rerio"
          },
          mod {
            {
              subtype other,
              subname "Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1st strand
 cDNA was primed with a Not I - oligo(dT)15 primer [5
'pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT3'], on mRNA from pooled 26
 somite zebrafish embryos; double-stranded cDNA was ligated to Sal I adaptors
 (BRL), digested with Not I and cloned into the Not I and Sal I sites of the
 pSPORT1 vector (BRL). Library was constructed by Matthew Clark (Lehrach lab;
 ICRF, London and  Max Planck Institut fuer Molekulare Genetik, Berlin) and
 was not biochemically normalised.  70,000 clones from this library were
 arrayed on high density filters and subsequently screened by oligonucleotide
 hybridization fingerprinting to identify unique or minimally redundant clones
 for more intensive analysis."
            }
          },
          lineage "Eukaryota; Metazoa; Chordata; Craniata; Vertebrata;
 Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
 Cypriniformes; Cyprinidae; Danio",
          gcode 1,
          mgcode 2,
          div "VRT"
        }
      },
      subtype {
        {
          subtype clone,
          name "23C8"
        },
        {
          subtype clone-lib,
          name "LIBEST_000899 Zebrafish ICRFzfls"
        },
        {
          subtype sex,
          name "mixed"
        },
        {
          subtype tissue-type,
          name "pooled 26-somite embryos"
        },
        {
          subtype lab-host,
          name "XL1-blue MRF"
        }
      }
    }
  },
  inst {
    repr raw,
    mol rna,
    length 362,
    strand ss,
    seq-data ncbi2na 'EFFFFFE1ED20B13F73FF3CB3330F92F011E34FDE83BEFB50247D0083
8277001EE9832EF0C17A13E7F8DF303E74144793B014EE34F4EEC3D535D48A99EFD3A3BE8BAF4B
F77E785B8D4AED37FABBAD149D316A5D051795B515EE4F70'H
  },
  annot {
    {
      desc {
        name "NCBI_GPIPE"
      },
      data ftable {
        {
          data region "alignable",
          location int {
            from 13,
            to 340,
            strand minus,
            id gi 2225005
          }
        },
        {
          data region "polya",
          location int {
            from 2,
            to 12,
            strand minus,
            id gi 2225005
          },
          qual {
            {
              qual "method",
              val "FindPolyTail"
            }
          }
        }
      }
    }
  }
}
Seq-entry ::= seq {
  id {
    general {
      db "dbEST",
      tag id 1216970
    },
    genbank {
      accession "AA566587",
      version 1
    },
    gi 2339219
  },
  descr {
    molinfo {
      biomol mRNA,
      tech est
    },
    title "ZF-ES108 zebrafish embryonic (subtraction) Danio rerio cDNA clone
 ZF-ES108 3' end.",
    create-date std {
      year 1997,
      month 8,
      day 20
    },
    update-date std {
      year 1998,
      month 8,
      day 25
    },
    source {
      org {
        taxname "Danio rerio",
        common "zebrafish",
        db {
          {
            db "taxon",
            tag id 7955
          }
        },
        orgname {
          name binomial {
            genus "Danio",
            species "rerio"
          },
          mod {
            {
              subtype other,
              subname "Organ: embryo; Vector: Lambda Uni-ZAP XR; Site_1:
 EcoRI; Site_2: XhoI; mRNA was purified from mixed stages of zebrafish embryos
 from 6  hours postfertilization to hatching.  cDNA was unidirectionally
 cloned  into the lambda ZAPII vector using the Stratagene's Lambda Uni-ZAP XR
  cDNA cloning system. "
            }
          },
          lineage "Eukaryota; Metazoa; Chordata; Craniata; Vertebrata;
 Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
 Cypriniformes; Cyprinidae; Danio",
          gcode 1,
          mgcode 2,
          div "VRT"
        }
      },
      subtype {
        {
          subtype clone,
          name "ZF-ES108"
        },
        {
          subtype clone-lib,
          name "LIBEST_000970 zebrafish embryonic (subtraction)"
        },
        {
          subtype tissue-type,
          name "embryonic"
        },
        {
          subtype dev-stage,
          name "mixed from 6 hours postfertilization to hatching"
        }
      }
    }
  },
  inst {
    repr raw,
    mol rna,
    length 239,
    strand ss,
    seq-data ncbi2na '7BBC4BBC04E4AFB2784F310083C7B0FD3DFB8139492C9C0D1D0AFF2B
C02C4EEC4BFDFFDF55FFCECBECDEC38FAF87E50C03AFB0FD17C0000000000760'H
  },
  annot {
    {
      desc {
        name "NCBI_GPIPE"
      },
      data ftable {
        {
          data region "alignable",
          location int {
            from 0,
            to 212,
            strand plus,
            id gi 2339219
          }
        },
        {
          data region "polya",
          location int {
            from 213,
            to 233,
            strand plus,
            id gi 2339219
          },
          qual {
            {
              qual "method",
              val "FindPolyTail"
            }
          }
        }
      }
    }
  }
}
Seq-entry ::= seq {
  id {
    general {
      db "dbEST",
      tag id 1864336
    },
    genbank {
      accession "AI106017",
      version 1
    },
    gi 3461120
  },
  descr {
    molinfo {
      biomol mRNA,
      tech est
    },
    title "DB04e11_p1 ZF adult heart library Danio rerio cDNA 5 prime.",
    create-date std {
      year 1998,
      month 8,
      day 25
    },
    update-date std {
      year 1998,
      month 8,
      day 25
    },
    source {
      org {
        taxname "Danio rerio",
        common "zebrafish",
        db {
          {
            db "taxon",
            tag id 7955
          }
        },
        orgname {
          name binomial {
            genus "Danio",
            species "rerio"
          },
          mod {
            {
              subtype strain,
              subname "AB"
            },
            {
              subtype other,
              subname "Organ: heart; Vector: LambdaZAPII; Site_1: EcoR1;
 Site_2: Xho1"
            }
          },
          lineage "Eukaryota; Metazoa; Chordata; Craniata; Vertebrata;
 Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
 Cypriniformes; Cyprinidae; Danio",
          gcode 1,
          mgcode 2,
          div "VRT"
        }
      },
      subtype {
        {
          subtype clone-lib,
          name "LIBEST_001401 ZF adult heart library"
        },
        {
          subtype sex,
          name "mixed"
        },
        {
          subtype tissue-type,
          name "myocardium, endocardium, vessel"
        },
        {
          subtype dev-stage,
          name "adult"
        },
        {
          subtype lab-host,
          name "E. coli XL1 Blue"
        }
      }
    }
  },
  inst {
    repr raw,
    mol rna,
    length 251,
    strand ss,
    seq-data ncbi4na '81188414218188F11421282111414214141214888228421424248211
441411241411411411148441442211411414442122844188414284111242214228428428221244
221421212882482142128114112112114228214282284414222182221812414888184421811182
8428412211118111288288848122124111111110'H
  },
  annot {
    {
      desc {
        name "NCBI_GPIPE"
      },
      data ftable {
        {
          data region "alignable",
          location int {
            from 0,
            to 242,
            strand plus,
            id gi 3461120
          }
        },
        {
          data region "polya",
          location int {
            from 243,
            to 250,
            strand plus,
            id gi 3461120
          },
          qual {
            {
              qual "method",
              val "FindPolyTail"
            }
          }
        }
      }
    }
  }
}