1LOCUS       NC_001422               5386 bp ss-DNA     circular PHG 09-JUL-2002
2DEFINITION  Coliphage phiX174, complete genome.
3ACCESSION   NC_001422
4VERSION     NC_001422.1  GI:9626372
5KEYWORDS    .
6SOURCE      coliphage phiX174.
7  ORGANISM  coliphage phiX174
8            Viruses; ssDNA viruses; Microviridae; Microvirus.
9REFERENCE   1  (bases 1047 to 1094)
10  AUTHORS   Ziff,E.B., Sedat,J.W. and Galibert,F.
11  TITLE     Determination of the nucleotide sequence of a fragment of
12            bacteriophage phiX 174 DNA
13  JOURNAL   Nature New Biol. 241 (106), 34-37 (1973)
14  MEDLINE   73161741
15   PUBMED   4349156
16REFERENCE   2  (bases 2370 to 2421)
17  AUTHORS   Robertson,H.D., Barrell,B.G., Weith,H.L. and Donelson,J.E.
18  TITLE     Isolation and sequence analysis of a ribosome-protected fragment
19            from bacteriophage phiX 174 DNA
20  JOURNAL   Nature New Biol. 241 (106), 38-40 (1973)
21  MEDLINE   73161742
22   PUBMED   4572838
23REFERENCE   3  (bases 2370 to 2420)
24  AUTHORS   Barrell,B.G., Weith,H.L., Donelson,J.E. and Robertson,H.D.
25  TITLE     Sequence analysis of the ribosome-protected bacteriophase phiX174
26            DNA fragment containing the gene G initiation site
27  JOURNAL   J. Mol. Biol. 92 (3), 377-393 (1975)
28  MEDLINE   75192039
29   PUBMED   1095758
30REFERENCE   4  (bases 2365 to 2591)
31  AUTHORS   Air,G.M., Blackburn,E.H., Sanger,F. and Coulson,A.R.
32  TITLE     The nucleotide and amino acid sequences of the N (5') terminal
33            region of gene G of bacteriophage phiphiX 174
34  JOURNAL   J. Mol. Biol. 96 (4), 703-719 (1975)
35  MEDLINE   76072037
36   PUBMED   1081600
37REFERENCE   5  (bases 2263 to 2421)
38  AUTHORS   Fiddes,J.C.
39  TITLE     Nucleotide sequence of the intercistronic region between genes G
40            and F in bacteriophage phiX174 DNA
41  JOURNAL   J. Mol. Biol. 107 (1), 1-24 (1976)
42  MEDLINE   77074135
43   PUBMED   826639
44REFERENCE   6  (bases 4137 to 4207)
45  AUTHORS   Mansfeld,A.D., Vereijken,J.M. and Jansz,H.S.
46  TITLE     The nucleotide sequence of a DNA fragment, 71 base pairs in length,
47            near the origin of DNA replication of bacteriophage 0X174
48  JOURNAL   Nucleic Acids Res. 3 (10), 2827-2844 (1976)
49  MEDLINE   77057432
50   PUBMED   995652
51REFERENCE   7  (bases 730 to 903)
52  AUTHORS   Blackburn,E.H.
53  TITLE     Transcription and sequence analysis of a fragment of bacteriophage
54            phiX174 DNA
55  JOURNAL   J. Mol. Biol. 107 (4), 417-431 (1976)
56  MEDLINE   77074161
57   PUBMED   826641
58REFERENCE   8  (bases 1017 to 1081)
59  AUTHORS   Sedat,J., Ziff,E. and Galibert,F.
60  TITLE     Direct determination of DNA nucleotide sequences. Structure of
61            large specific fragments of bacteriophage phiX174 DNA
62  JOURNAL   J. Mol. Biol. 107 (4), 391-416 (1976)
63  MEDLINE   77074160
64   PUBMED   1003475
65REFERENCE   9  (bases 1017 to 1762)
66  AUTHORS   Air,G.M., Blackburn,E.H., Coulson,A.R., Galibert,F., Sanger,F.,
67            Sedat,J.W. and Ziff,E.B.
68  TITLE     Gene F of bacteriophage phiX174. Correlation of nucleotide
69            sequences from the DNA and amino acid sequences from the gene
70            product
71  JOURNAL   J. Mol. Biol. 107 (4), 445-458 (1976)
72  MEDLINE   77074163
73   PUBMED   1088826
74REFERENCE   10 (bases 2395 to 2922)
75  AUTHORS   Air,G.M., Sanger,F. and Coulson,A.R.
76  TITLE     Nucleotide and amino acid sequences of gene G of omegaX174
77  JOURNAL   J. Mol. Biol. 108 (3), 519-533 (1976)
78  MEDLINE   77121207
79   PUBMED   1088827
80REFERENCE   11 (bases 5022 to 5132)
81  AUTHORS   Brown,N.L. and Smith,M.
82  TITLE     DNA sequence of a region of the phi X174 genome coding for a
83            ribosome binding site
84  JOURNAL   Nature 265 (5596), 695-698 (1977)
85  MEDLINE   77171176
86   PUBMED   859573
87REFERENCE   12 (bases 5346 to 5386; 1 to 159)
88  AUTHORS   Smith,M., Brown,N.L., Air,G.M., Barrell,B.G., Coulson,A.R.,
89            Hutchison,C.A. III and Sanger,F.
90  TITLE     DNA sequence at the C termini of the overlapping genes A and B in
91            bacteriophage phi X174
92  JOURNAL   Nature 265 (5596), 702-705 (1977)
93  MEDLINE   77171178
94   PUBMED   859575
95REFERENCE   13 (bases 1 to 5375)
96  AUTHORS   Sanger,F., Air,G.M., Barrell,B.G., Brown,N.L., Coulson,A.R.,
97            Fiddes,C.A., Hutchison,C.A., Slocombe,P.M. and Smith,M.
98  TITLE     Nucliotide sequence of bacteriophage phi X174 DNA
99  JOURNAL   Nature 265 (5596), 687-695 (1977)
100  MEDLINE   77171175
101   PUBMED   870828
102REFERENCE   14 (bases 4505 to 5374)
103  AUTHORS   Brown,N.L. and Smith,M.
104  TITLE     The sequence of a region of bacteriophage phiX174 DNA coding for
105            parts of genes A and B
106  JOURNAL   J. Mol. Biol. 116 (1), 1-28 (1977)
107  MEDLINE   78069208
108   PUBMED   592379
109REFERENCE   15 (sites)
110  AUTHORS   Fiddes,J.C.
111  TITLE     The nucleotide sequence of a viral DNA
112  JOURNAL   Sci. Am. 237 (6), 54-67 (1977)
113  MEDLINE   78054683
114   PUBMED   929160
115REFERENCE   16 (bases 1 to 5386)
116  AUTHORS   Sanger,F., Coulson,A.R., Friedmann,T., Air,G.M., Barrell,B.G.,
117            Brown,N.L., Fiddes,J.C., Hutchison,C.A. III, Slocombe,P.M. and
118            Smith,M.
119  TITLE     The nucleotide sequence of bacteriophage phiX174
120  JOURNAL   J. Mol. Biol. 125 (2), 225-246 (1978)
121  MEDLINE   79091185
122   PUBMED   731693
123REFERENCE   17 (bases 1290 to 1302; 1340 to 1430; 1510 to 1570; 1600 to 1750)
124  AUTHORS   Air,G.M., Coulson,A.R., Fiddes,J.C., Friedmann,T., Hutchison,C.A.
125            III, Sanger,F., Slocombe,P.M. and Smith,A.J.
126  TITLE     Nucleotide sequence of the F protein coding region of bacteriophage
127            phiX174 and the amino acid sequence of its product
128  JOURNAL   J. Mol. Biol. 125 (2), 247-254 (1978)
129  MEDLINE   79091186
130   PUBMED   731694
131REFERENCE   18 (bases 4256 to 4317)
132  AUTHORS   Langeveld,S.A., van Mansfeld,A.D., de Winter,J.M. and Weisbeek,P.J.
133  TITLE     Cleavage of single-stranded DNA by the A and A* proteins of
134            bacteriophage phi X174
135  JOURNAL   Nucleic Acids Res. 7 (8), 2177-2188 (1979)
136  MEDLINE   80101074
137   PUBMED   160544
138REFERENCE   19 (bases 4248 to 4332)
139  AUTHORS   Heidekamp,F., Langeveld,S.A., Baas,P.D. and Jansz,H.S.
140  TITLE     Studies of the recognition sequence of phi X174 gene A protein.
141            Cleavage site of phi X gene A protein in St-1 RFI DNA
142  JOURNAL   Nucleic Acids Res. 8 (9), 2009-2021 (1980)
143  MEDLINE   81053861
144   PUBMED   6253953
145REFERENCE   20 (bases 436 to 490; 630 to 669; 930 to 979)
146  AUTHORS   Takeshita,M., Kappen,L.S., Grollman,A.P., Eisenberg,M. and
147            Goldberg,I.H.
148  TITLE     Strand scission of deoxyribonucleic acid by neocarzinostatin,
149            auromomycin, and bleomycin: studies on base release and nucleotide
150            sequence specificity
151  JOURNAL   Biochemistry (N.Y.) 20 (26), 7599-7606 (1981)
152  MEDLINE   82113627
153   PUBMED   6173064
154REFERENCE   21 (bases 449 to 482; 504 to 598; 1047 to 1111)
155  AUTHORS   Ueda,K., Morita,J. and Komano,T.
156  TITLE     Sequence specificity of heat-labile sites in DNA induced by
157            mitomycin C
158  JOURNAL   Biochemistry (N.Y.) 23 (8), 1634-1640 (1984)
159  MEDLINE   84203526
160   PUBMED   6232949
161REFERENCE   22 (bases 1064 to 1757)
162  AUTHORS   Merville,M.P., Piette,J., Lopez,M., Decuyper,J. and van de Vorst,A.
163  TITLE     Termination sites of the in vitro DNA synthesis on single-stranded
164            DNA photosensitized by promazines
165  JOURNAL   J. Biol. Chem. 259 (24), 15069-15077 (1984)
166  MEDLINE   85079985
167   PUBMED   6239864
168REFERENCE   23 (bases 2380 to 2512; 2593 to 2786; 2788 to 2947)
169  AUTHORS   Air,G.M., Els,M.C., Brown,L.E., Laver,W.G. and Webster,R.G.
170  TITLE     Location of antigenic sites on the three-dimensional structure of
171            the influenza N2 virus neuraminidase
172  JOURNAL   Virology 145 (2), 237-248 (1985)
173  MEDLINE   85274373
174   PUBMED   2411049
175COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
176            reference sequence was derived from J02482.
177            [8]  intermittent sequences.
178            [15]  review; discussion of complete genome.
179            Double checked with sumex tape.
180            Single-stranded circular DNA which codes for eleven proteins.
181            Replicative form is duplex, icosahedron, related to s13 & g4. [21]
182            indicates that mitomycin C reduced with sodium borohydride induced
183            heat-labile sites in DNA most preferentially at dinucleotide
184            sequence 'gt' (especially 'Pu-g-t').
185            Bacteriophage phi-X174 single stranded DNA molecules were
186            irradiated with near UV light in the presence of promazine
187            derivatives, after priming with restriction fragments or synthetic
188            primers [22].  The resulting DNA fragments were used as templates
189            for in vitro complementary chain synthesis by E.coli DNA polymerase
190            I [22].  More than 90% of the observed chain terminations were
191            mapped one nucleotide before a guanine residue [22].  Photoreaction
192            occurred more predominantly with guanine residues localized in
193            single-stranded parts of the genome [22].  These same guanine
194            residues could also be damaged when the reaction was performed in
195            the dark, in the presence of promazine cation radicals [22].
196FEATURES             Location/Qualifiers
197     source          1..5386
198                     /organism="coliphage phiX174"
199                     /specific_host="Escherichia coli"
200                     /db_xref="taxon:10847"
201     CDS             join(3981..5386,1..136)
202                     /codon_start=1
203                     /transl_table=11
204                     /product="rf replication, viral strand synthesis protein"
205                     /protein_id="NP_040703.1"
206                     /db_xref="GI:9626373"
207                     /translation="MVRSYYPSECHADYFDFERIEALKPAIEACGISTLSQSPMLGFH
208                     KQMDNRIKLLEEILSFRMQGVEFDNGDMYVDGHKAASDVRDEFVSVTEKLMDELAQCY
209                     NVLPQLDINNTIDHRPEGDEKWFLENEKTVTQFCRKLAAERPLKDIRDEYNYPKKKGI
210                     KDECSRLLEASTMKSRRGFAIQRLMNAMRQAHADGWFIVFDTLTLADDRLEAFYDNPN
211                     ALRDYFRDIGRMVLAAEGRKANDSHADCYQYFCVPEYGTANGRLHFHAVHFMRTLPTG
212                     SVDPNFGRRVRNRRQLNSLQNTWPYGYSMPIAVRYTQDAFSRSGWLWPVDAKGEPLKA
213                     TSYMAVGFYVAKYVNKKSDMDLAAKGLGAKEWNNSLKTKLSLLPKKLFRIRMSRNFGM
214                     KMLTMTNLSTECLIQLTKLGYDATPFNQILKQNAKREMRLRLGKVTVADVLAAQPVTT
215                     NLLKFMRASIKMIGVSNLQSFIASMTQKLTLSDISDESKNYLDKAGITTACLRIKSKW
216                     TAGGK"
217     CDS             join(4497..5386,1..136)
218                     /codon_start=1
219                     /transl_table=11
220                     /product="shut off host DNA synthesis protein"
221                     /protein_id="NP_040704.1"
222                     /db_xref="GI:9626374"
223                     /translation="MKSRRGFAIQRLMNAMRQAHADGWFIVFDTLTLADDRLEAFYDN
224                     PNALRDYFRDIGRMVLAAEGRKANDSHADCYQYFCVPEYGTANGRLHFHAVHFMRTLP
225                     TGSVDPNFGRRVRNRRQLNSLQNTWPYGYSMPIAVRYTQDAFSRSGWLWPVDAKGEPL
226                     KATSYMAVGFYVAKYVNKKSDMDLAAKGLGAKEWNNSLKTKLSLLPKKLFRIRMSRNF
227                     GMKMLTMTNLSTECLIQLTKLGYDATPFNQILKQNAKREMRLRLGKVTVADVLAAQPV
228                     TTNLLKFMRASIKMIGVSNLQSFIASMTQKLTLSDISDESKNYLDKAGITTACLRIKS
229                     KWTAGGK"
230     CDS             join(5075..5386,1..51)
231                     /codon_start=1
232                     /transl_table=11
233                     /product="capsid morphogenesis protein"
234                     /protein_id="NP_040705.1"
235                     /db_xref="GI:9626375"
236                     /translation="MEQLTKNQAVATSQEAVQNQNEPQLRDENAHNDKSVHGVLNPTY
237                     QAGLRRDAVQPDIEAERKKRDEIEAGKSYCSRRFGGATCDDKSAQIYARFDKNDWRIQ
238                     PAEFYRFHDAEVNTFGYF"
239     variation       23
240                     /note="c in wt; t in am18 and am35 [14]"
241     variation       25
242                     /note="g in wt; c in ts116 [14]"
243     CDS             51..221
244                     /codon_start=1
245                     /transl_table=11
246                     /product="gene K protein"
247                     /protein_id="NP_040706.1"
248                     /db_xref="GI:9626376"
249                     /translation="MSRKIILIKQELLLLVYELNRSGLLAENEKIRPILAQLEKLLLC
250                     DLSPSTNDSVKN"
251     variation       57
252                     /note="c in wt; t in am6 [14]"
253     variation       117
254                     /note="g in wt; a in am6 [14]"
255     CDS             133..393
256                     /codon_start=1
257                     /transl_table=11
258                     /product="DNA maturation protein"
259                     /protein_id="NP_040707.1"
260                     /db_xref="GI:9626377"
261                     /translation="MRKFDLSLRSSRSSYFATFRHQLTILSKTDALDEEKWLNMLGTF
262                     VKDWFRYESHFVHGRDSLVDILKERGLLSESDAVQPLIGKKS"
263     mRNA            358..3975
264                     /note="mRNA (major alt.)"
265     mRNA            358..991
266                     /note="mRNA (minor alt.)"
267     CDS             390..848
268                     /codon_start=1
269                     /transl_table=11
270                     /product="capsid morphogenesis protein"
271                     /protein_id="NP_040708.1"
272                     /db_xref="GI:9626378"
273                     /translation="MSQVTEQSVRFQTALASIKLIQASAVLDLTEDDFDFLTSNKVWI
274                     ATDRSRARRCVEACVYGTLDFVGYPRFPAPVEFIAAVIAYYVHPVNIQTACLIMEGAE
275                     FTENIINGVERPVKAAELFAFTLRVRAGNTDVLTDAEENVRQKLRAEGVM"
276     CDS             568..843
277                     /codon_start=1
278                     /transl_table=11
279                     /product="cell lysis protein"
280                     /protein_id="NP_040709.1"
281                     /db_xref="GI:9626379"
282                     /translation="MVRWTLWDTLAFLLLLSLLLPSLLIMFIPSTFKRPVSSWKALNL
283                     RKTLLMASSVRLKPLNCSRLPCVYAQETLTFLLTQKKTCVKNYVRKE"
284     CDS             848..964
285                     /codon_start=1
286                     /transl_table=11
287                     /product="core protein, DNA condensation protein"
288                     /protein_id="NP_040710.1"
289                     /db_xref="GI:9626380"
290                     /translation="MSKGKKRSGARPGRPQPLRGTKGKRKGARLWYVGGQQF"
291     CDS             1001..2284
292                     /codon_start=1
293                     /transl_table=11
294                     /product="major coat protein"
295                     /protein_id="NP_040711.1"
296                     /db_xref="GI:9626381"
297                     /translation="MSNIQTGAERMPHDLSHLGFLAGQIGRLITISTTPVIAGDSFEM
298                     DAVGALRLSPLRRGLAIDSTVDIFTFYVPHRHVYGEQWIKFMKDGVNATPLPTVNTTG
299                     YIDHAAFLGTINPDTNKIPKHLFQGYLNIYNNYFKAPWMPDRTEANPNELNQDDARYG
300                     FRCCHLKNIWTAPLPPETELSRQMTTSTTSIDIMGLQAAYANLHTDQERDYFMQRYHD
301                     VISSFGGKTSYDADNRPLLVMRSNLWASGYDVDGTDQTSLGQFSGRVQQTYKHSVPRF
302                     FVPEHGTMFTLALVRFPPTATKEIQYLNAKGALTYTDIAGDPVLYGNLPPREISMKDV
303                     FRSGDSSKKFKIAEGQWYRYAPSYVSPAYHLLEGFPFIQEPPSGDLQERVLIRHHDYD
304                     QCFQSVQLLQWNSQVKFNVTVYRNLPTTRDSIMTS"
305     CDS             2395..2922
306                     /codon_start=1
307                     /transl_table=11
308                     /product="major spike protein"
309                     /protein_id="NP_040712.1"
310                     /db_xref="GI:9626382"
311                     /translation="MFQTFISRHNSNFFSDKLVLTSVTPASSAPVLQTPKATSSTLYF
312                     DSLTVNAGNGGFLHCIQMDTSVNAANQVVSVGADIAFDADPKFFACLVRFESSSVPTT
313                     LPTAYDVYPLNGRHDGGYYTVKDCVTIDVLPRTPGNNVYVGFMVWSNFTATKCRGLVS
314                     LNQVIKEIICLQPLK"
315     CDS             2931..3917
316                     /codon_start=1
317                     /transl_table=11
318                     /product="minor spike protein, adsorption"
319                     /protein_id="NP_040713.1"
320                     /db_xref="GI:9626383"
321                     /translation="MFGAIAGGIASALAGGAMSKLFGGGQKAASGGIQGDVLATDNNT
322                     VGMGDAGIKSAIQGSNVPNPDEAAPSFVSGAMAKAGKGLLEGTLQAGTSAVSDKLLDL
323                     VGLGGKSAADKGKDTRDYLAAAFPELNAWERAGADASSAGMVDAGFENQKELTKMQLD
324                     NQKEIAEMQNETQKEIAGIQSATSRQNTKDQVYAQNEMLAYQQKESTARVASIMENTN
325                     LSKQQQVSEIMRQMLTQAQTAGQYFTNDQIKEMTRKVSAEVDLVHQQTQNQRYGSSHI
326                     GATAKDISNVVTDAASGVVDIFHGIDKAVADTWNNFWKDGKADGIGSNLSRK"
327     misc_feature    3962
328                     /note="transcription start site"
329     rep_origin      4306
330                     /note="origin of viral strand synthesis"
331     misc_feature    4899
332                     /note="transcription start site"
333BASE COUNT     1291 a   1157 c   1254 g   1684 t
334ORIGIN
335        1 gagttttatc gcttccatga cgcagaagtt aacactttcg gatatttctg atgagtcgaa
336       61 aaattatctt gataaagcag gaattactac tgcttgttta cgaattaaat cgaagtggac
337      121 tgctggcgga aaatgagaaa attcgaccta tccttgcgca gctcgagaag ctcttacttt
338      181 gcgacctttc gccatcaact aacgattctg tcaaaaactg acgcgttgga tgaggagaag
339      241 tggcttaata tgcttggcac gttcgtcaag gactggttta gatatgagtc acattttgtt
340      301 catggtagag attctcttgt tgacatttta aaagagcgtg gattactatc tgagtccgat
341      361 gctgttcaac cactaatagg taagaaatca tgagtcaagt tactgaacaa tccgtacgtt
342      421 tccagaccgc tttggcctct attaagctca ttcaggcttc tgccgttttg gatttaaccg
343      481 aagatgattt cgattttctg acgagtaaca aagtttggat tgctactgac cgctctcgtg
344      541 ctcgtcgctg cgttgaggct tgcgtttatg gtacgctgga ctttgtggga taccctcgct
345      601 ttcctgctcc tgttgagttt attgctgccg tcattgctta ttatgttcat cccgtcaaca
346      661 ttcaaacggc ctgtctcatc atggaaggcg ctgaatttac ggaaaacatt attaatggcg
347      721 tcgagcgtcc ggttaaagcc gctgaattgt tcgcgtttac cttgcgtgta cgcgcaggaa
348      781 acactgacgt tcttactgac gcagaagaaa acgtgcgtca aaaattacgt gcggaaggag
349      841 tgatgtaatg tctaaaggta aaaaacgttc tggcgctcgc cctggtcgtc cgcagccgtt
350      901 gcgaggtact aaaggcaagc gtaaaggcgc tcgtctttgg tatgtaggtg gtcaacaatt
351      961 ttaattgcag gggcttcggc cccttacttg aggataaatt atgtctaata ttcaaactgg
352     1021 cgccgagcgt atgccgcatg acctttccca tcttggcttc cttgctggtc agattggtcg
353     1081 tcttattacc atttcaacta ctccggttat cgctggcgac tccttcgaga tggacgccgt
354     1141 tggcgctctc cgtctttctc cattgcgtcg tggccttgct attgactcta ctgtagacat
355     1201 ttttactttt tatgtccctc atcgtcacgt ttatggtgaa cagtggatta agttcatgaa
356     1261 ggatggtgtt aatgccactc ctctcccgac tgttaacact actggttata ttgaccatgc
357     1321 cgcttttctt ggcacgatta accctgatac caataaaatc cctaagcatt tgtttcaggg
358     1381 ttatttgaat atctataaca actattttaa agcgccgtgg atgcctgacc gtaccgaggc
359     1441 taaccctaat gagcttaatc aagatgatgc tcgttatggt ttccgttgct gccatctcaa
360     1501 aaacatttgg actgctccgc ttcctcctga gactgagctt tctcgccaaa tgacgacttc
361     1561 taccacatct attgacatta tgggtctgca agctgcttat gctaatttgc atactgacca
362     1621 agaacgtgat tacttcatgc agcgttacca tgatgttatt tcttcatttg gaggtaaaac
363     1681 ctcttatgac gctgacaacc gtcctttact tgtcatgcgc tctaatctct gggcatctgg
364     1741 ctatgatgtt gatggaactg accaaacgtc gttaggccag ttttctggtc gtgttcaaca
365     1801 gacctataaa cattctgtgc cgcgtttctt tgttcctgag catggcacta tgtttactct
366     1861 tgcgcttgtt cgttttccgc ctactgcgac taaagagatt cagtacctta acgctaaagg
367     1921 tgctttgact tataccgata ttgctggcga ccctgttttg tatggcaact tgccgccgcg
368     1981 tgaaatttct atgaaggatg ttttccgttc tggtgattcg tctaagaagt ttaagattgc
369     2041 tgagggtcag tggtatcgtt atgcgccttc gtatgtttct cctgcttatc accttcttga
370     2101 aggcttccca ttcattcagg aaccgccttc tggtgatttg caagaacgcg tacttattcg
371     2161 ccaccatgat tatgaccagt gtttccagtc cgttcagttg ttgcagtgga atagtcaggt
372     2221 taaatttaat gtgaccgttt atcgcaatct gccgaccact cgcgattcaa tcatgacttc
373     2281 gtgataaaag attgagtgtg aggttataac gccgaagcgg taaaaatttt aatttttgcc
374     2341 gctgaggggt tgaccaagcg aagcgcggta ggttttctgc ttaggagttt aatcatgttt
375     2401 cagactttta tttctcgcca taattcaaac tttttttctg ataagctggt tctcacttct
376     2461 gttactccag cttcttcggc acctgtttta cagacaccta aagctacatc gtcaacgtta
377     2521 tattttgata gtttgacggt taatgctggt aatggtggtt ttcttcattg cattcagatg
378     2581 gatacatctg tcaacgccgc taatcaggtt gtttctgttg gtgctgatat tgcttttgat
379     2641 gccgacccta aattttttgc ctgtttggtt cgctttgagt cttcttcggt tccgactacc
380     2701 ctcccgactg cctatgatgt ttatcctttg aatggtcgcc atgatggtgg ttattatacc
381     2761 gtcaaggact gtgtgactat tgacgtcctt ccccgtacgc cgggcaataa cgtttatgtt
382     2821 ggtttcatgg tttggtctaa ctttaccgct actaaatgcc gcggattggt ttcgctgaat
383     2881 caggttatta aagagattat ttgtctccag ccacttaagt gaggtgattt atgtttggtg
384     2941 ctattgctgg cggtattgct tctgctcttg ctggtggcgc catgtctaaa ttgtttggag
385     3001 gcggtcaaaa agccgcctcc ggtggcattc aaggtgatgt gcttgctacc gataacaata
386     3061 ctgtaggcat gggtgatgct ggtattaaat ctgccattca aggctctaat gttcctaacc
387     3121 ctgatgaggc cgcccctagt tttgtttctg gtgctatggc taaagctggt aaaggacttc
388     3181 ttgaaggtac gttgcaggct ggcacttctg ccgtttctga taagttgctt gatttggttg
389     3241 gacttggtgg caagtctgcc gctgataaag gaaaggatac tcgtgattat cttgctgctg
390     3301 catttcctga gcttaatgct tgggagcgtg ctggtgctga tgcttcctct gctggtatgg
391     3361 ttgacgccgg atttgagaat caaaaagagc ttactaaaat gcaactggac aatcagaaag
392     3421 agattgccga gatgcaaaat gagactcaaa aagagattgc tggcattcag tcggcgactt
393     3481 cacgccagaa tacgaaagac caggtatatg cacaaaatga gatgcttgct tatcaacaga
394     3541 aggagtctac tgctcgcgtt gcgtctatta tggaaaacac caatctttcc aagcaacagc
395     3601 aggtttccga gattatgcgc caaatgctta ctcaagctca aacggctggt cagtatttta
396     3661 ccaatgacca aatcaaagaa atgactcgca aggttagtgc tgaggttgac ttagttcatc
397     3721 agcaaacgca gaatcagcgg tatggctctt ctcatattgg cgctactgca aaggatattt
398     3781 ctaatgtcgt cactgatgct gcttctggtg tggttgatat ttttcatggt attgataaag
399     3841 ctgttgccga tacttggaac aatttctgga aagacggtaa agctgatggt attggctcta
400     3901 atttgtctag gaaataaccg tcaggattga caccctccca attgtatgtt ttcatgcctc
401     3961 caaatcttgg aggctttttt atggttcgtt cttattaccc ttctgaatgt cacgctgatt
402     4021 attttgactt tgagcgtatc gaggctctta aacctgctat tgaggcttgt ggcatttcta
403     4081 ctctttctca atccccaatg cttggcttcc ataagcagat ggataaccgc atcaagctct
404     4141 tggaagagat tctgtctttt cgtatgcagg gcgttgagtt cgataatggt gatatgtatg
405     4201 ttgacggcca taaggctgct tctgacgttc gtgatgagtt tgtatctgtt actgagaagt
406     4261 taatggatga attggcacaa tgctacaatg tgctccccca acttgatatt aataacacta
407     4321 tagaccaccg ccccgaaggg gacgaaaaat ggtttttaga gaacgagaag acggttacgc
408     4381 agttttgccg caagctggct gctgaacgcc ctcttaagga tattcgcgat gagtataatt
409     4441 accccaaaaa gaaaggtatt aaggatgagt gttcaagatt gctggaggcc tccactatga
410     4501 aatcgcgtag aggctttgct attcagcgtt tgatgaatgc aatgcgacag gctcatgctg
411     4561 atggttggtt tatcgttttt gacactctca cgttggctga cgaccgatta gaggcgtttt
412     4621 atgataatcc caatgctttg cgtgactatt ttcgtgatat tggtcgtatg gttcttgctg
413     4681 ccgagggtcg caaggctaat gattcacacg ccgactgcta tcagtatttt tgtgtgcctg
414     4741 agtatggtac agctaatggc cgtcttcatt tccatgcggt gcactttatg cggacacttc
415     4801 ctacaggtag cgttgaccct aattttggtc gtcgggtacg caatcgccgc cagttaaata
416     4861 gcttgcaaaa tacgtggcct tatggttaca gtatgcccat cgcagttcgc tacacgcagg
417     4921 acgctttttc acgttctggt tggttgtggc ctgttgatgc taaaggtgag ccgcttaaag
418     4981 ctaccagtta tatggctgtt ggtttctatg tggctaaata cgttaacaaa aagtcagata
419     5041 tggaccttgc tgctaaaggt ctaggagcta aagaatggaa caactcacta aaaaccaagc
420     5101 tgtcgctact tcccaagaag ctgttcagaa tcagaatgag ccgcaacttc gggatgaaaa
421     5161 tgctcacaat gacaaatctg tccacggagt gcttaatcca acttaccaag ctgggttacg
422     5221 acgcgacgcc gttcaaccag atattgaagc agaacgcaaa aagagagatg agattgaggc
423     5281 tgggaaaagt tactgtagcc gacgttttgg cggcgcaacc tgtgacgaca aatctgctca
424     5341 aatttatgcg cgcttcgata aaaatgattg gcgtatccaa cctgca
425//
426