1% BFST(1) bFst (vcflib) | bFst (VCF statistics) 2% Erik Garrison and vcflib contributors 3 4# NAME 5 6**bFst** 7 8# SYNOPSIS 9 10**bFst** --target 0,1,2,3,4,5,6,7 --background 11,12,13,16,17,19,22 --file my.vcf --deltaaf 0.1 11 12# DESCRIPTION 13 14**bFst** is a Bayesian approach to Fst. Importantly **bFst** accounts for genotype uncertainty in the model using genotype likelihoods. For a more detailed description see: `A Bayesian approach to inferring population structure from dominant markers' by Holsinger et al. Molecular Ecology Vol 11, issue 7 2002. The likelihood function has been modified to use genotype likelihoods provided by variant callers. There are five free parameters estimated in the model: each subpopulation's allele frequency and Fis (fixation index, within each subpopulation), a free parameter for the total population's allele frequency, and Fst. 15 16 17 18# OPTIONS 19 20``` 21 22 23Output : 11 columns : 24 1. Seqid 25 2. Position 26 3. Observed allele frequency in target. 27 4. Estimated allele frequency in target. 28 5. Observed allele frequency in background. 29 6. Estimated allele frequency in background. 30 7. Observed allele frequency combined. 31 8. Estimated allele frequency in combined. 32 9. ML estimate of Fst (mean) 33 10. Lower bound of the 95% credible interval 34 11. Upper bound of the 95% credible interval 35 36required: t,target -- a zero bases comma separated list of target individuals corrisponding to VCF columns 37required: b,background -- a zero bases comma separated list of background individuals corrisponding to VCF columns 38required: f,file a -- a proper formatted VCF file. the FORMAT field MUST contain "PL" 39required: d,deltaaf -- skip sites were the difference in allele frequency is less than deltaaf 40 41 42Type: statistics 43 44``` 45 46 47 48 49 50# EXIT VALUES 51 52**0** 53: Success 54 55**not 0** 56: Failure 57 58# SEE ALSO 59 60 61 62[vcflib](./vcflib.md)(1) 63 64 65 66# OTHER 67 68## Source code 69 70[bFst.cpp](https://github.com/vcflib/vcflib/blob/master/src/bFst.cpp) 71 72# LICENSE 73 74Copyright 2011-2020 (C) Erik Garrison and vcflib contributors. MIT licensed. 75 76<!-- 77 Created with ./scripts/bin2md.rb scripts/bin2md-template.erb 78--> 79