1<multi-test> 2 <plugin_primer_3> 3 <plugin_primer_3_in 4 5 PRIMER_SEQUENCE_ID="SP01-21.txt" 6 SEQUENCE="GAACATGTGTGGCATTGTGTATACACATATGTGTTGTGTATAAATGTTGTGTCTTCANTGTGTCTTGTGGGTGTGTCTGTTAAACATGTGTGGTATTGTGCATATACATGTGTGTTGTGTATGGATGTAGCTTCTTGACTGTGTGTTGTGTGTGTGTGTCTGTTGTGTATATACACGTGTGTTCTATGTANATGTGTCTAGAGTGTATGTTGTGTGTGTGTGTAGATC" 7 PRIMER_FIRST_BASE_INDEX="1" 8 PRIMER_SELF_ANY="12" 9 PRIMER_MAX_POLY_X="100" 10 PRIMER_INTERNAL_OLIGO_MAX_POLY_X="100" 11 PRIMER_SELF_END="8" 12 PRIMER_FILE_FLAG="1" 13 14 /> 15 <plugin_primer_3_out 16 PRIMER_PAIRS_NUMBER="5" 17 PRIMER_PAIR_PENALTY="5.1035" 18 PRIMER_LEFT_PENALTY="2.224770" 19 PRIMER_RIGHT_PENALTY="2.878720" 20 PRIMER_LEFT_SEQUENCE="GAACATGTGTGGCATTGTGT" 21 PRIMER_RIGHT_SEQUENCE="CAACAGACACACACACACAACA" 22 PRIMER_LEFT="1,20" 23 PRIMER_RIGHT="165,22" 24 PRIMER_LEFT_TM="57.775" 25 PRIMER_RIGHT_TM="59.121" 26 PRIMER_LEFT_GC_PERCENT="45.000" 27 PRIMER_RIGHT_GC_PERCENT="45.455" 28 PRIMER_LEFT_SELF_ANY="6.00" 29 PRIMER_RIGHT_SELF_ANY="1.00" 30 PRIMER_LEFT_SELF_END="0.00" 31 PRIMER_RIGHT_SELF_END="0.00" 32 PRIMER_LEFT_END_STABILITY="6.4000" 33 PRIMER_RIGHT_END_STABILITY="7.0000" 34 PRIMER_PAIR_COMPL_ANY="6.00" 35 PRIMER_PAIR_COMPL_END="5.00" 36 PRIMER_PRODUCT_SIZE="165" 37 PRIMER_PAIR_PENALTY_1="5.1035" 38 PRIMER_LEFT_1_PENALTY="2.224770" 39 PRIMER_RIGHT_1_PENALTY="2.878720" 40 PRIMER_LEFT_1_SEQUENCE="GAACATGTGTGGCATTGTGT" 41 PRIMER_RIGHT_1_SEQUENCE="CACAACAGACACACACACACAA" 42 PRIMER_LEFT_1="1,20" 43 PRIMER_RIGHT_1="167,22" 44 PRIMER_LEFT_1_TM="57.775" 45 PRIMER_RIGHT_1_TM="59.121" 46 PRIMER_LEFT_1_GC_PERCENT="45.000" 47 PRIMER_RIGHT_1_GC_PERCENT="45.455" 48 PRIMER_LEFT_1_SELF_ANY="6.00" 49 PRIMER_RIGHT_1_SELF_ANY="1.00" 50 PRIMER_LEFT_1_SELF_END="0.00" 51 PRIMER_RIGHT_1_SELF_END="0.00" 52 PRIMER_LEFT_1_END_STABILITY="6.4000" 53 PRIMER_RIGHT_1_END_STABILITY="7.0000" 54 PRIMER_PAIR_1_COMPL_ANY="8.00" 55 PRIMER_PAIR_1_COMPL_END="6.00" 56 PRIMER_PRODUCT_SIZE_1="167" 57 PRIMER_PAIR_PENALTY_2="5.1035" 58 PRIMER_LEFT_2_PENALTY="2.224770" 59 PRIMER_RIGHT_2_PENALTY="2.878720" 60 PRIMER_LEFT_2_SEQUENCE="GAACATGTGTGGCATTGTGT" 61 PRIMER_RIGHT_2_SEQUENCE="CACACACACAACACACAGTCAA" 62 PRIMER_LEFT_2="1,20" 63 PRIMER_RIGHT_2="156,22" 64 PRIMER_LEFT_2_TM="57.775" 65 PRIMER_RIGHT_2_TM="59.121" 66 PRIMER_LEFT_2_GC_PERCENT="45.000" 67 PRIMER_RIGHT_2_GC_PERCENT="45.455" 68 PRIMER_LEFT_2_SELF_ANY="6.00" 69 PRIMER_RIGHT_2_SELF_ANY="3.00" 70 PRIMER_LEFT_2_SELF_END="0.00" 71 PRIMER_RIGHT_2_SELF_END="0.00" 72 PRIMER_LEFT_2_END_STABILITY="6.4000" 73 PRIMER_RIGHT_2_END_STABILITY="6.7000" 74 PRIMER_PAIR_2_COMPL_ANY="7.00" 75 PRIMER_PAIR_2_COMPL_END="6.00" 76 PRIMER_PRODUCT_SIZE_2="156" 77 PRIMER_PAIR_PENALTY_3="5.2887" 78 PRIMER_LEFT_3_PENALTY="2.224770" 79 PRIMER_RIGHT_3_PENALTY="3.063978" 80 PRIMER_LEFT_3_SEQUENCE="GAACATGTGTGGCATTGTGT" 81 PRIMER_RIGHT_3_SEQUENCE="ACAACAGACACACACACACAACA" 82 PRIMER_LEFT_3="1,20" 83 PRIMER_RIGHT_3="166,23" 84 PRIMER_LEFT_3_TM="57.775" 85 PRIMER_RIGHT_3_TM="60.064" 86 PRIMER_LEFT_3_GC_PERCENT="45.000" 87 PRIMER_RIGHT_3_GC_PERCENT="43.478" 88 PRIMER_LEFT_3_SELF_ANY="6.00" 89 PRIMER_RIGHT_3_SELF_ANY="1.00" 90 PRIMER_LEFT_3_SELF_END="0.00" 91 PRIMER_RIGHT_3_SELF_END="0.00" 92 PRIMER_LEFT_3_END_STABILITY="6.4000" 93 PRIMER_RIGHT_3_END_STABILITY="7.0000" 94 PRIMER_PAIR_3_COMPL_ANY="7.00" 95 PRIMER_PAIR_3_COMPL_END="5.00" 96 PRIMER_PRODUCT_SIZE_3="166" 97 PRIMER_PAIR_PENALTY_4="5.2887" 98 PRIMER_LEFT_4_PENALTY="2.224770" 99 PRIMER_RIGHT_4_PENALTY="3.063978" 100 PRIMER_LEFT_4_SEQUENCE="GAACATGTGTGGCATTGTGT" 101 PRIMER_RIGHT_4_SEQUENCE="CAACAGACACACACACACAACAC" 102 PRIMER_LEFT_4="1,20" 103 PRIMER_RIGHT_4="165,23" 104 PRIMER_LEFT_4_TM="57.775" 105 PRIMER_RIGHT_4_TM="60.064" 106 PRIMER_LEFT_4_GC_PERCENT="45.000" 107 PRIMER_RIGHT_4_GC_PERCENT="47.826" 108 PRIMER_LEFT_4_SELF_ANY="6.00" 109 PRIMER_RIGHT_4_SELF_ANY="1.00" 110 PRIMER_LEFT_4_SELF_END="0.00" 111 PRIMER_RIGHT_4_SELF_END="0.00" 112 PRIMER_LEFT_4_END_STABILITY="6.4000" 113 PRIMER_RIGHT_4_END_STABILITY="6.4000" 114 PRIMER_PAIR_4_COMPL_ANY="6.00" 115 PRIMER_PAIR_4_COMPL_END="6.00" 116 PRIMER_PRODUCT_SIZE_4="165" 117 /> 118 </plugin_primer_3> 119</multi-test> 120