1######################################## 2# Program: restrict 3# Rundate: Wed 18 Feb 2009 11:01:39 4# Commandline: restrict 5# [-sequence] /Users/tjc/foo.embl 6# -auto 7# -limit y 8# -enzymes HincII,hinfI,ppiI,hindiii 9# -outfile /private/tmp/circular_genome57650.txt 10# Report_format: table 11# Report_file: /private/tmp/circular_genome57650.txt 12######################################## 13 14#======================================= 15# 16# Sequence: from: 1 to: 41243 17# HitCount: 158 18# 19# Minimum cuts per enzyme: 1 20# Maximum cuts per enzyme: 2000000000 21# Minimum length of recognition site: 4 22# Blunt ends allowed 23# Sticky ends allowed 24# DNA is linear 25# Ambiguities allowed 26# 27#======================================= 28 29 Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 30 81 86 + HindIII AAGCTT 81 85 . . 31 140 144 + HinfI GANTC 140 143 . . 32 40956 40960 + HinfI GANTC 40956 40959 . . 33 41036 41041 + HindII GTYRAC 41038 41038 . . 34 35#--------------------------------------- 36#--------------------------------------- 37 38#--------------------------------------- 39# Total_sequences: 1 40# Total_hitcount: 158 41#--------------------------------------- 42