1########################################
2# Program: restrict
3# Rundate: Wed 18 Feb 2009 11:01:39
4# Commandline: restrict
5#    [-sequence] /Users/tjc/foo.embl
6#    -auto
7#    -limit y
8#    -enzymes HincII,hinfI,ppiI,hindiii
9#    -outfile /private/tmp/circular_genome57650.txt
10# Report_format: table
11# Report_file: /private/tmp/circular_genome57650.txt
12########################################
13
14#=======================================
15#
16# Sequence:      from: 1   to: 41243
17# HitCount: 158
18#
19# Minimum cuts per enzyme: 1
20# Maximum cuts per enzyme: 2000000000
21# Minimum length of recognition site: 4
22# Blunt ends allowed
23# Sticky ends allowed
24# DNA is linear
25# Ambiguities allowed
26#
27#=======================================
28
29  Start     End  Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev
30     81      86       + HindIII     AAGCTT               81     85         .         .
31    140     144       + HinfI       GANTC               140    143         .         .
32  40956   40960       + HinfI       GANTC             40956  40959         .         .
33  41036   41041       + HindII      GTYRAC            41038  41038         .         .
34
35#---------------------------------------
36#---------------------------------------
37
38#---------------------------------------
39# Total_sequences: 1
40# Total_hitcount: 158
41#---------------------------------------
42