1 2<a name="input.1"></a> 3<h3>Input files for usage example </h3> 4 5'tsw:gcn4_yeast' is a sequence entry in the example protein database 'tsw' 6<p> 7<p><h3>Database entry: tsw:gcn4_yeast</h3> 8<table width="90%"><tr><td bgcolor="#FFCCFF"> 9<pre> 10ID GCN4_YEAST Reviewed; 281 AA. 11AC P03069; D3DLN9; P03068; Q70D88; Q70D91; Q70D96; Q70D99; Q70DA0; 12AC Q96UT3; 13DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot. 14DT 21-JUL-1986, sequence version 1. 15DT 13-JUN-2012, entry version 139. 16DE RecName: Full=General control protein GCN4; 17DE AltName: Full=Amino acid biosynthesis regulatory protein; 18GN Name=GCN4; Synonyms=AAS3, ARG9; OrderedLocusNames=YEL009C; 19OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). 20OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; 21OC Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. 22OX NCBI_TaxID=559292; 23RN [1] 24RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. 25RX MEDLINE=85038531; PubMed=6387704; DOI=10.1073/pnas.81.20.6442; 26RA Hinnebusch A.G.; 27RT "Evidence for translational regulation of the activator of general 28RT amino acid control in yeast."; 29RL Proc. Natl. Acad. Sci. U.S.A. 81:6442-6446(1984). 30RN [2] 31RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. 32RX MEDLINE=84298088; PubMed=6433345; DOI=10.1073/pnas.81.16.5096; 33RA Thireos G., Penn M.D., Greer H.; 34RT "5' untranslated sequences are required for the translational control 35RT of a yeast regulatory gene."; 36RL Proc. Natl. Acad. Sci. U.S.A. 81:5096-5100(1984). 37RN [3] 38RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS PRO-24; SER-62; 39RP ALA-82; ALA-91; ALA-125 AND GLU-196. 40RC STRAIN=CLIB 219, CLIB 382, CLIB 388, CLIB 410, CLIB 413, CLIB 556, 41RC CLIB 630, CLIB 95, K1, R12, R13, Sigma 1278B, YIIc12, and YIIc17; 42RX PubMed=15087486; DOI=10.1093/nar/gkh529; 43RA Leh-Louis V., Wirth B., Despons L., Wain-Hobson S., Potier S., 44RA Souciet J.-L.; 45RT "Differential evolution of the Saccharomyces cerevisiae DUP240 46RT paralogs and implication of recombination in phylogeny."; 47RL Nucleic Acids Res. 32:2069-2078(2004). 48RN [4] 49RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. 50RC STRAIN=ATCC 204511 / S288c / AB972; 51RX MEDLINE=97313264; PubMed=9169868; 52RA Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., 53RA Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., 54RA Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., 55RA Hunicke-Smith S., Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H., 56RA Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., 57RA Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., 58RA Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.; 59RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome V."; 60 61 62<font color=red> [Part of this file has been deleted for brevity]</font> 63 64FT /FTId=PRO_0000076490. 65FT DOMAIN 253 274 Leucine-zipper. 66FT DNA_BIND 231 249 Basic motif. 67FT REGION 89 100 Required for transcriptional activation. 68FT REGION 106 125 Required for transcriptional activation. 69FT MOD_RES 17 17 Phosphoserine. 70FT MOD_RES 165 165 Phosphothreonine; by PHO85. 71FT MOD_RES 218 218 Phosphoserine. 72FT VARIANT 24 24 S -> P (in strain: CLIB 219). 73FT VARIANT 62 62 P -> S (in strain: CLIB 630 haplotype 74FT Ha2). 75FT VARIANT 82 82 T -> A (in strain: CLIB 556 haplotype 76FT Ha1). 77FT VARIANT 91 91 D -> A (in strain: CLIB 95, CLIB 219, 78FT CLIB 382, CLIB 388, CLIB 410, CLIB 413, 79FT CLIB 556, CLIB 630, K1, R12, R13 80FT haplotype Ha2, Sigma 1278B haplotype Ha1, 81FT YIIc12 and YIIc17). 82FT VARIANT 125 125 D -> A (in strain: CLIB 556 haplotype 83FT Ha1). 84FT VARIANT 196 196 D -> E (in strain: CLIB 388, CLIB 410, 85FT CLIB 413, CLIB 630 haplotype Ha1, K1, 86FT YIIc12 haplotype Ha2 and YIIc17 haplotype 87FT Ha1). 88FT MUTAGEN 97 98 FF->AA: Reduces transcriptional 89FT activation activity; when associated with 90FT A-107; A-110; A-113; A-120; A-123 and A- 91FT 124. 92FT MUTAGEN 107 107 M->A: Reduces transcriptional activation 93FT activity; when associated with A-97; A- 94FT 98; A-110; A-113; A-120; A-123 and A-124. 95FT MUTAGEN 110 110 Y->A: Reduces transcriptional activation 96FT activity; when associated with A-97; A- 97FT 98; A-107; A-113; A-120; A-123 and A-124. 98FT MUTAGEN 113 113 L->A: Reduces transcriptional activation 99FT activity; when associated with A-97; A- 100FT 98; A-107; A-110; A-120; A-123 and A-124. 101FT MUTAGEN 120 124 WTSLF->ATSAA: Reduces transcriptional 102FT activation activity; when associated with 103FT A-97; A-98; A-107; A-110 and A-113. 104FT CONFLICT 239 281 ARRSRARKLQRMKQLEDKVEELLSKNYHLENEVARLKKLVG 105FT ER -> PGVLVRESCKE (in Ref. 2; AAA65521). 106FT HELIX 230 248 107FT HELIX 251 280 108SQ SEQUENCE 281 AA; 31310 MW; 2ED1B8E35D509578 CRC64; 109 MSEYQPSLFA LNPMGFSPLD GSKSTNENVS ASTSTAKPMV GQLIFDKFIK TEEDPIIKQD 110 TPSNLDFDFA LPQTATAPDA KTVLPIPELD DAVVESFFSS STDSTPMFEY ENLEDNSKEW 111 TSLFDNDIPV TTDDVSLADK AIESTEEVSL VPSNLEVSTT SFLPTPVLED AKLTQTRKVK 112 KPNSVVKKSH HVGKDDESRL DHLGVVAYNR KQRSIPLSPI VPESSDPAAL KRARNTEAAR 113 RSRARKLQRM KQLEDKVEEL LSKNYHLENE VARLKKLVGE R 114// 115</pre> 116</td></tr></table><p> 117