1LOCUS       PX1CG                   5386 bp ss-DNA     circular PHG 16-JUN-2004
2DEFINITION  Coliphage phi-X174, complete genome.
3ACCESSION   J02482 M10348 M10379 M10714 M10749 M10750 M10866 M10867 M24859
4            V01128
5VERSION     J02482.1  GI:216019
6KEYWORDS    .
7SOURCE      Enterobacteria phage phiX174
8  ORGANISM  Enterobacteria phage phiX174
9            Viruses; ssDNA viruses; Microviridae; Microvirus.
10REFERENCE   1  (bases 1047 to 1094)
11  AUTHORS   Ziff,E.B., Sedat,J.W. and Galibert,F.
12  TITLE     Determination of the nucleotide sequence of a fragment of
13            bacteriophage phiX 174 DNA
14  JOURNAL   Nature New Biol. 241 (106), 34-37 (1973)
15   PUBMED   4349156
16REFERENCE   2  (bases 2370 to 2421)
17  AUTHORS   Robertson,H.D., Barrell,B.G., Weith,H.L. and Donelson,J.E.
18  TITLE     Isolation and sequence analysis of a ribosome-protected fragment
19            from bacteriophage phiX 174 DNA
20  JOURNAL   Nature New Biol. 241 (106), 38-40 (1973)
21   PUBMED   4572838
22REFERENCE   3  (bases 2370 to 2420)
23  AUTHORS   Barrell,B.G., Weith,H.L., Donelson,J.E. and Robertson,H.D.
24  TITLE     Sequence analysis of the ribosome-protected bacteriophase phiX174
25            DNA fragment containing the gene G initiation site
26  JOURNAL   J. Mol. Biol. 92 (3), 377-393 (1975)
27   PUBMED   1095758
28REFERENCE   4  (bases 2365 to 2591)
29  AUTHORS   Air,G.M., Blackburn,E.H., Sanger,F. and Coulson,A.R.
30  TITLE     The nucleotide and amino acid sequences of the N (5') terminal
31            region of gene G of bacteriophage phiphiX 174
32  JOURNAL   J. Mol. Biol. 96 (4), 703-719 (1975)
33   PUBMED   1081600
34REFERENCE   5  (bases 2263 to 2421)
35  AUTHORS   Fiddes,J.C.
36  TITLE     Nucleotide sequence of the intercistronic region between genes G
37            and F in bacteriophage phiX174 DNA
38  JOURNAL   J. Mol. Biol. 107 (1), 1-24 (1976)
39   PUBMED   826639
40REFERENCE   6  (bases 1017 to 1081)
41  AUTHORS   Sedat,J., Ziff,E. and Galibert,F.
42  TITLE     Direct determination of DNA nucleotide sequences. Structure of
43            large specific fragments of bacteriophage phiX174 DNA
44  JOURNAL   J. Mol. Biol. 107 (4), 391-416 (1976)
45   PUBMED   1003475
46REFERENCE   7  (bases 730 to 903)
47  AUTHORS   Blackburn,E.H.
48  TITLE     Transcription and sequence analysis of a fragment of bacteriophage
49            phiX174 DNA
50  JOURNAL   J. Mol. Biol. 107 (4), 417-431 (1976)
51   PUBMED   826641
52REFERENCE   8  (bases 1017 to 1762)
53  AUTHORS   Air,G.M., Blackburn,E.H., Coulson,A.R., Galibert,F., Sanger,F.,
54            Sedat,J.W. and Ziff,E.B.
55  TITLE     Gene F of bacteriophage phiX174. Correlation of nucleotide
56            sequences from the DNA and amino acid sequences from the gene
57            product
58  JOURNAL   J. Mol. Biol. 107 (4), 445-458 (1976)
59   PUBMED   1088826
60REFERENCE   9  (bases 2395 to 2922)
61  AUTHORS   Air,G.M., Sanger,F. and Coulson,A.R.
62  TITLE     Nucleotide and amino acid sequences of gene G of omegaX174
63  JOURNAL   J. Mol. Biol. 108 (3), 519-533 (1976)
64   PUBMED   1088827
65REFERENCE   10 (bases 4137 to 4207)
66  AUTHORS   Mansfeld,A.D., Vereijken,J.M. and Jansz,H.S.
67  TITLE     The nucleotide sequence of a DNA fragment, 71 base pairs in length,
68            near the origin of DNA replication of bacteriophage 0X174
69  JOURNAL   Nucleic Acids Res. 3 (10), 2827-2844 (1976)
70   PUBMED   995652
71REFERENCE   11 (bases 4505 to 5374)
72  AUTHORS   Brown,N.L. and Smith,M.
73  TITLE     The sequence of a region of bacteriophage phiX174 DNA coding for
74            parts of genes A and B
75  JOURNAL   J. Mol. Biol. 116 (1), 1-28 (1977)
76   PUBMED   592379
77REFERENCE   12 (bases 1 to 5375)
78  AUTHORS   Sanger,F., Air,G.M., Barrell,B.G., Brown,N.L., Coulson,A.R.,
79            Fiddes,C.A., Hutchison,C.A., Slocombe,P.M. and Smith,M.
80  TITLE     Nucleotide sequence of bacteriophage phi X174 DNA
81  JOURNAL   Nature 265 (5596), 687-695 (1977)
82   PUBMED   870828
83REFERENCE   13 (bases 5022 to 5132)
84  AUTHORS   Brown,N.L. and Smith,M.
85  TITLE     DNA sequence of a region of the phi X174 genome coding for a
86            ribosome binding site
87  JOURNAL   Nature 265 (5596), 695-698 (1977)
88   PUBMED   859573
89REFERENCE   14 (bases 5346 to 5386; 1 to 159)
90  AUTHORS   Smith,M., Brown,N.L., Air,G.M., Barrell,B.G., Coulson,A.R.,
91            Hutchison,C.A. III and Sanger,F.
92  TITLE     DNA sequence at the C termini of the overlapping genes A and B in
93            bacteriophage phi X174
94  JOURNAL   Nature 265 (5596), 702-705 (1977)
95   PUBMED   859575
96REFERENCE   15 (sites)
97  AUTHORS   Fiddes,J.C.
98  TITLE     The nucleotide sequence of a viral DNA
99  JOURNAL   Sci. Am. 237 (6), 54-67 (1977)
100   PUBMED   929160
101REFERENCE   16 (bases 1 to 5386)
102  AUTHORS   Sanger,F., Coulson,A.R., Friedmann,T., Air,G.M., Barrell,B.G.,
103            Brown,N.L., Fiddes,J.C., Hutchison,C.A. III, Slocombe,P.M. and
104            Smith,M.
105  TITLE     The nucleotide sequence of bacteriophage phiX174
106  JOURNAL   J. Mol. Biol. 125 (2), 225-246 (1978)
107   PUBMED   731693
108REFERENCE   17 (bases 1290 to 1302; 1340 to 1430; 1510 to 1570; 1600 to 1750)
109  AUTHORS   Air,G.M., Coulson,A.R., Fiddes,J.C., Friedmann,T., Hutchison,C.A.
110            III, Sanger,F., Slocombe,P.M. and Smith,A.J.
111  TITLE     Nucleotide sequence of the F protein coding region of bacteriophage
112            phiX174 and the amino acid sequence of its product
113  JOURNAL   J. Mol. Biol. 125 (2), 247-254 (1978)
114   PUBMED   731694
115REFERENCE   18 (bases 4256 to 4317)
116  AUTHORS   Langeveld,S.A., van Mansfeld,A.D., de Winter,J.M. and Weisbeek,P.J.
117  TITLE     Cleavage of single-stranded DNA by the A and A* proteins of
118            bacteriophage phi X174
119  JOURNAL   Nucleic Acids Res. 7 (8), 2177-2188 (1979)
120   PUBMED   160544
121REFERENCE   19 (bases 4248 to 4332)
122  AUTHORS   Heidekamp,F., Langeveld,S.A., Baas,P.D. and Jansz,H.S.
123  TITLE     Studies of the recognition sequence of phi X174 gene A protein.
124            Cleavage site of phi X gene A protein in St-1 RFI DNA
125  JOURNAL   Nucleic Acids Res. 8 (9), 2009-2021 (1980)
126   PUBMED   6253953
127REFERENCE   20 (bases 436 to 490; 630 to 669; 930 to 979)
128  AUTHORS   Takeshita,M., Kappen,L.S., Grollman,A.P., Eisenberg,M. and
129            Goldberg,I.H.
130  TITLE     Strand scission of deoxyribonucleic acid by neocarzinostatin,
131            auromomycin, and bleomycin: studies on base release and nucleotide
132            sequence specificity
133  JOURNAL   Biochemistry 20 (26), 7599-7606 (1981)
134   PUBMED   6173064
135REFERENCE   22 (bases 1064 to 1757)
136  AUTHORS   Merville,M.P., Piette,J., Lopez,M., Decuyper,J. and van de Vorst,A.
137  TITLE     Termination sites of the in vitro DNA synthesis on single-stranded
138            DNA photosensitized by promazines
139  JOURNAL   J. Biol. Chem. 259 (24), 15069-15077 (1984)
140   PUBMED   6239864
141REFERENCE   23 (bases 2380 to 2512; 2593 to 2786; 2788 to 2947)
142  AUTHORS   Air,G.M., Els,M.C., Brown,L.E., Laver,W.G. and Webster,R.G.
143  TITLE     Location of antigenic sites on the three-dimensional structure of
144            the influenza N2 virus neuraminidase
145  JOURNAL   Virology 145 (2), 237-248 (1985)
146   PUBMED   2411049
147REFERENCE   24 (bases 449 to 482; 504 to 598; 1047 to 1111)
148  AUTHORS   Ueda,K., Morita,J. and Komano,T.
149  TITLE     Sequence specificity of heat-labile sites in DNA induced by
150            mitomycin C
151  JOURNAL   Biochemistry 23 (8), 1634-1640 (1984)
152   PUBMED   6232949
153COMMENT     On Apr 28, 2004 this sequence version replaced gi:15535.
154            [8]  intermittent sequences.
155            [15]  review; discussion of complete genome.
156            Double checked with sumex tape.
157            Single-stranded circular DNA which codes for eleven proteins.
158            Replicative form is duplex, icosahedron, related to s13 & g4. [21]
159            indicates that mitomycin C reduced with sodium borohydride induced
160            heat-labile sites in DNA most preferentially at dinucleotide
161            sequence 'gt' (especially 'Pu-g-t').
162            Bacteriophage phi-X174 single stranded DNA molecules were
163            irradiated with near UV light in the presence of promazine
164            derivatives, after priming with restriction fragments or synthetic
165            primers [22].  The resulting DNA fragments were used as templates
166            for in vitro complementary chain synthesis by E.coli DNA polymerase
167            I [22].  More than 90% of the observed chain terminations were
168            mapped one nucleotide before a guanine residue [22].  Photoreaction
169            occurred more predominantly with guanine residues localized in
170            single-stranded parts of the genome [22].  These same guanine
171            residues could also be damaged when the reaction was performed in
172            the dark, in the presence of promazine cation radicals [22].
173FEATURES             Location/Qualifiers
174     source          1..5386
175                     /organism="Enterobacteria phage phiX174"
176                     /mol_type="genomic DNA"
177                     /db_xref="taxon:10847"
178     CDS             join(3981..5386,1..136)
179                     /function="viral strand synthesis"
180                     /note="rf replication"
181                     /codon_start=1
182                     /transl_table=11
183                     /product="A"
184                     /protein_id="AAA32570.1"
185                     /db_xref="GI:216020"
186                     /translation="MVRSYYPSECHADYFDFERIEALKPAIEACGISTLSQSPMLGFH
187                     KQMDNRIKLLEEILSFRMQGVEFDNGDMYVDGHKAASDVRDEFVSVTEKLMDELAQCY
188                     NVLPQLDINNTIDHRPEGDEKWFLENEKTVTQFCRKLAAERPLKDIRDEYNYPKKKGI
189                     KDECSRLLEASTMKSRRGFAIQRLMNAMRQAHADGWFIVFDTLTLADDRLEAFYDNPN
190                     ALRDYFRDIGRMVLAAEGRKANDSHADCYQYFCVPEYGTANGRLHFHAVHFMRTLPTG
191                     SVDPNFGRRVRNRRQLNSLQNTWPYGYSMPIAVRYTQDAFSRSGWLWPVDAKGEPLKA
192                     TSYMAVGFYVAKYVNKKSDMDLAAKGLGAKEWNNSLKTKLSLLPKKLFRIRMSRNFGM
193                     KMLTMTNLSTECLIQLTKLGYDATPFNQILKQNAKREMRLRLGKVTVADVLAAQPVTT
194                     NLLKFMRASIKMIGVSNLQSFIASMTQKLTLSDISDESKNYLDKAGITTACLRIKSKW
195                     TAGGK"
196     CDS             join(4497..5386,1..136)
197                     /function="shut off host DNA synthesis"
198                     /codon_start=1
199                     /transl_table=11
200                     /product="A*"
201                     /protein_id="AAA32571.1"
202                     /db_xref="GI:216021"
203                     /translation="MKSRRGFAIQRLMNAMRQAHADGWFIVFDTLTLADDRLEAFYDN
204                     PNALRDYFRDIGRMVLAAEGRKANDSHADCYQYFCVPEYGTANGRLHFHAVHFMRTLP
205                     TGSVDPNFGRRVRNRRQLNSLQNTWPYGYSMPIAVRYTQDAFSRSGWLWPVDAKGEPL
206                     KATSYMAVGFYVAKYVNKKSDMDLAAKGLGAKEWNNSLKTKLSLLPKKLFRIRMSRNF
207                     GMKMLTMTNLSTECLIQLTKLGYDATPFNQILKQNAKREMRLRLGKVTVADVLAAQPV
208                     TTNLLKFMRASIKMIGVSNLQSFIASMTQKLTLSDISDESKNYLDKAGITTACLRIKS
209                     KWTAGGK"
210     CDS             join(5075..5386,1..51)
211                     /function="capsid morphogenesis"
212                     /codon_start=1
213                     /transl_table=11
214                     /product="B"
215                     /protein_id="AAA32572.1"
216                     /db_xref="GI:216022"
217                     /translation="MEQLTKNQAVATSQEAVQNQNEPQLRDENAHNDKSVHGVLNPTY
218                     QAGLRRDAVQPDIEAERKKRDEIEAGKSYCSRRFGGATCDDKSAQIYARFDKNDWRIQ
219                     PAEFYRFHDAEVNTFGYF"
220     variation       23
221                     /note="in am18 and am35 [14]"
222                     /replace="t"
223     variation       25
224                     /note="ts116 [14]"
225                     /replace="c"
226     CDS             51..221
227                     /codon_start=1
228                     /transl_table=11
229                     /product="K"
230                     /protein_id="AAA32573.1"
231                     /db_xref="GI:216023"
232                     /translation="MSRKIILIKQELLLLVYELNRSGLLAENEKIRPILAQLEKLLLC
233                     DLSPSTNDSVKN"
234     variation       57
235                     /note="am6 [14]"
236                     /replace="c"
237     variation       117
238                     /note="am6 [14]"
239                     /replace="a"
240     CDS             133..393
241                     /note="DNA maturation"
242                     /codon_start=1
243                     /transl_table=11
244                     /product="C"
245                     /protein_id="AAA32574.1"
246                     /db_xref="GI:216024"
247                     /translation="MRKFDLSLRSSRSSYFATFRHQLTILSKTDALDEEKWLNMLGTF
248                     VKDWFRYESHFVHGRDSLVDILKERGLLSESDAVQPLIGKKS"
249     mRNA            358..3975
250                     /product="major transcript"
251     mRNA            358..991
252                     /product="minor transcript"
253     CDS             390..848
254                     /function="capsid morphogenesis"
255                     /codon_start=1
256                     /transl_table=11
257                     /product="D"
258                     /protein_id="AAA32575.1"
259                     /db_xref="GI:216025"
260                     /translation="MSQVTEQSVRFQTALASIKLIQASAVLDLTEDDFDFLTSNKVWI
261                     ATDRSRARRCVEACVYGTLDFVGYPRFPAPVEFIAAVIAYYVHPVNIQTACLIMEGAE
262                     FTENIINGVERPVKAAELFAFTLRVRAGNTDVLTDAEENVRQKLRAEGVM"
263     CDS             568..843
264                     /function="cell lysis"
265                     /codon_start=1
266                     /transl_table=11
267                     /product="E"
268                     /protein_id="AAA32576.1"
269                     /db_xref="GI:216026"
270                     /translation="MVRWTLWDTLAFLLLLSLLLPSLLIMFIPSTFKRPVSSWKALNL
271                     RKTLLMASSVRLKPLNCSRLPCVYAQETLTFLLTQKKTCVKNYVRKE"
272     CDS             848..964
273                     /note="core protein; DNA condensation"
274                     /codon_start=1
275                     /transl_table=11
276                     /product="J"
277                     /protein_id="AAA32577.1"
278                     /db_xref="GI:216027"
279                     /translation="MSKGKKRSGARPGRPQPLRGTKGKRKGARLWYVGGQQF"
280     CDS             1001..2284
281                     /note="major coat protein"
282                     /codon_start=1
283                     /transl_table=11
284                     /product="F"
285                     /protein_id="AAA32578.1"
286                     /db_xref="GI:216028"
287                     /translation="MSNIQTGAERMPHDLSHLGFLAGQIGRLITISTTPVIAGDSFEM
288                     DAVGALRLSPLRRGLAIDSTVDIFTFYVPHRHVYGEQWIKFMKDGVNATPLPTVNTTG
289                     YIDHAAFLGTINPDTNKIPKHLFQGYLNIYNNYFKAPWMPDRTEANPNELNQDDARYG
290                     FRCCHLKNIWTAPLPPETELSRQMTTSTTSIDIMGLQAAYANLHTDQERDYFMQRYHD
291                     VISSFGGKTSYDADNRPLLVMRSNLWASGYDVDGTDQTSLGQFSGRVQQTYKHSVPRF
292                     FVPEHGTMFTLALVRFPPTATKEIQYLNAKGALTYTDIAGDPVLYGNLPPREISMKDV
293                     FRSGDSSKKFKIAEGQWYRYAPSYVSPAYHLLEGFPFIQEPPSGDLQERVLIRHHDYD
294                     QCFQSVQLLQWNSQVKFNVTVYRNLPTTRDSIMTS"
295     CDS             2395..2922
296                     /note="major spike protein"
297                     /codon_start=1
298                     /transl_table=11
299                     /product="G"
300                     /protein_id="AAA32579.1"
301                     /db_xref="GI:216029"
302                     /translation="MFQTFISRHNSNFFSDKLVLTSVTPASSAPVLQTPKATSSTLYF
303                     DSLTVNAGNGGFLHCIQMDTSVNAANQVVSVGADIAFDADPKFFACLVRFESSSVPTT
304                     LPTAYDVYPLNGRHDGGYYTVKDCVTIDVLPRTPGNNVYVGFMVWSNFTATKCRGLVS
305                     LNQVIKEIICLQPLK"
306     CDS             2931..3917
307                     /function="adsorption"
308                     /note="minor spike protein"
309                     /codon_start=1
310                     /transl_table=11
311                     /product="H"
312                     /protein_id="AAA32580.1"
313                     /db_xref="GI:216030"
314                     /translation="MFGAIAGGIASALAGGAMSKLFGGGQKAASGGIQGDVLATDNNT
315                     VGMGDAGIKSAIQGSNVPNPDEAAPSFVSGAMAKAGKGLLEGTLQAGTSAVSDKLLDL
316                     VGLGGKSAADKGKDTRDYLAAAFPELNAWERAGADASSAGMVDAGFENQKELTKMQLD
317                     NQKEIAEMQNETQKEIAGIQSATSRQNTKDQVYAQNEMLAYQQKESTARVASIMENTN
318                     LSKQQQVSEIMRQMLTQAQTAGQYFTNDQIKEMTRKVSAEVDLVHQQTQNQRYGSSHI
319                     GATAKDISNVVTDAASGVVDIFHGIDKAVADTWNNFWKDGKADGIGSNLSRK"
320     misc_feature    3962
321                     /note="transcription start site"
322     rep_origin      4306
323                     /note="origin of viral strand synthesis"
324     misc_feature    4899
325                     /note="transcription start site"
326ORIGIN
327        1 gagttttatc gcttccatga cgcagaagtt aacactttcg gatatttctg atgagtcgaa
328       61 aaattatctt gataaagcag gaattactac tgcttgttta cgaattaaat cgaagtggac
329      121 tgctggcgga aaatgagaaa attcgaccta tccttgcgca gctcgagaag ctcttacttt
330      181 gcgacctttc gccatcaact aacgattctg tcaaaaactg acgcgttgga tgaggagaag
331      241 tggcttaata tgcttggcac gttcgtcaag gactggttta gatatgagtc acattttgtt
332      301 catggtagag attctcttgt tgacatttta aaagagcgtg gattactatc tgagtccgat
333      361 gctgttcaac cactaatagg taagaaatca tgagtcaagt tactgaacaa tccgtacgtt
334      421 tccagaccgc tttggcctct attaagctca ttcaggcttc tgccgttttg gatttaaccg
335      481 aagatgattt cgattttctg acgagtaaca aagtttggat tgctactgac cgctctcgtg
336      541 ctcgtcgctg cgttgaggct tgcgtttatg gtacgctgga ctttgtggga taccctcgct
337      601 ttcctgctcc tgttgagttt attgctgccg tcattgctta ttatgttcat cccgtcaaca
338      661 ttcaaacggc ctgtctcatc atggaaggcg ctgaatttac ggaaaacatt attaatggcg
339      721 tcgagcgtcc ggttaaagcc gctgaattgt tcgcgtttac cttgcgtgta cgcgcaggaa
340      781 acactgacgt tcttactgac gcagaagaaa acgtgcgtca aaaattacgt gcggaaggag
341      841 tgatgtaatg tctaaaggta aaaaacgttc tggcgctcgc cctggtcgtc cgcagccgtt
342      901 gcgaggtact aaaggcaagc gtaaaggcgc tcgtctttgg tatgtaggtg gtcaacaatt
343      961 ttaattgcag gggcttcggc cccttacttg aggataaatt atgtctaata ttcaaactgg
344     1021 cgccgagcgt atgccgcatg acctttccca tcttggcttc cttgctggtc agattggtcg
345     1081 tcttattacc atttcaacta ctccggttat cgctggcgac tccttcgaga tggacgccgt
346     1141 tggcgctctc cgtctttctc cattgcgtcg tggccttgct attgactcta ctgtagacat
347     1201 ttttactttt tatgtccctc atcgtcacgt ttatggtgaa cagtggatta agttcatgaa
348     1261 ggatggtgtt aatgccactc ctctcccgac tgttaacact actggttata ttgaccatgc
349     1321 cgcttttctt ggcacgatta accctgatac caataaaatc cctaagcatt tgtttcaggg
350     1381 ttatttgaat atctataaca actattttaa agcgccgtgg atgcctgacc gtaccgaggc
351     1441 taaccctaat gagcttaatc aagatgatgc tcgttatggt ttccgttgct gccatctcaa
352     1501 aaacatttgg actgctccgc ttcctcctga gactgagctt tctcgccaaa tgacgacttc
353     1561 taccacatct attgacatta tgggtctgca agctgcttat gctaatttgc atactgacca
354     1621 agaacgtgat tacttcatgc agcgttacca tgatgttatt tcttcatttg gaggtaaaac
355     1681 ctcttatgac gctgacaacc gtcctttact tgtcatgcgc tctaatctct gggcatctgg
356     1741 ctatgatgtt gatggaactg accaaacgtc gttaggccag ttttctggtc gtgttcaaca
357     1801 gacctataaa cattctgtgc cgcgtttctt tgttcctgag catggcacta tgtttactct
358     1861 tgcgcttgtt cgttttccgc ctactgcgac taaagagatt cagtacctta acgctaaagg
359     1921 tgctttgact tataccgata ttgctggcga ccctgttttg tatggcaact tgccgccgcg
360     1981 tgaaatttct atgaaggatg ttttccgttc tggtgattcg tctaagaagt ttaagattgc
361     2041 tgagggtcag tggtatcgtt atgcgccttc gtatgtttct cctgcttatc accttcttga
362     2101 aggcttccca ttcattcagg aaccgccttc tggtgatttg caagaacgcg tacttattcg
363     2161 ccaccatgat tatgaccagt gtttccagtc cgttcagttg ttgcagtgga atagtcaggt
364     2221 taaatttaat gtgaccgttt atcgcaatct gccgaccact cgcgattcaa tcatgacttc
365     2281 gtgataaaag attgagtgtg aggttataac gccgaagcgg taaaaatttt aatttttgcc
366     2341 gctgaggggt tgaccaagcg aagcgcggta ggttttctgc ttaggagttt aatcatgttt
367     2401 cagactttta tttctcgcca taattcaaac tttttttctg ataagctggt tctcacttct
368     2461 gttactccag cttcttcggc acctgtttta cagacaccta aagctacatc gtcaacgtta
369     2521 tattttgata gtttgacggt taatgctggt aatggtggtt ttcttcattg cattcagatg
370     2581 gatacatctg tcaacgccgc taatcaggtt gtttctgttg gtgctgatat tgcttttgat
371     2641 gccgacccta aattttttgc ctgtttggtt cgctttgagt cttcttcggt tccgactacc
372     2701 ctcccgactg cctatgatgt ttatcctttg aatggtcgcc atgatggtgg ttattatacc
373     2761 gtcaaggact gtgtgactat tgacgtcctt ccccgtacgc cgggcaataa cgtttatgtt
374     2821 ggtttcatgg tttggtctaa ctttaccgct actaaatgcc gcggattggt ttcgctgaat
375     2881 caggttatta aagagattat ttgtctccag ccacttaagt gaggtgattt atgtttggtg
376     2941 ctattgctgg cggtattgct tctgctcttg ctggtggcgc catgtctaaa ttgtttggag
377     3001 gcggtcaaaa agccgcctcc ggtggcattc aaggtgatgt gcttgctacc gataacaata
378     3061 ctgtaggcat gggtgatgct ggtattaaat ctgccattca aggctctaat gttcctaacc
379     3121 ctgatgaggc cgcccctagt tttgtttctg gtgctatggc taaagctggt aaaggacttc
380     3181 ttgaaggtac gttgcaggct ggcacttctg ccgtttctga taagttgctt gatttggttg
381     3241 gacttggtgg caagtctgcc gctgataaag gaaaggatac tcgtgattat cttgctgctg
382     3301 catttcctga gcttaatgct tgggagcgtg ctggtgctga tgcttcctct gctggtatgg
383     3361 ttgacgccgg atttgagaat caaaaagagc ttactaaaat gcaactggac aatcagaaag
384     3421 agattgccga gatgcaaaat gagactcaaa aagagattgc tggcattcag tcggcgactt
385     3481 cacgccagaa tacgaaagac caggtatatg cacaaaatga gatgcttgct tatcaacaga
386     3541 aggagtctac tgctcgcgtt gcgtctatta tggaaaacac caatctttcc aagcaacagc
387     3601 aggtttccga gattatgcgc caaatgctta ctcaagctca aacggctggt cagtatttta
388     3661 ccaatgacca aatcaaagaa atgactcgca aggttagtgc tgaggttgac ttagttcatc
389     3721 agcaaacgca gaatcagcgg tatggctctt ctcatattgg cgctactgca aaggatattt
390     3781 ctaatgtcgt cactgatgct gcttctggtg tggttgatat ttttcatggt attgataaag
391     3841 ctgttgccga tacttggaac aatttctgga aagacggtaa agctgatggt attggctcta
392     3901 atttgtctag gaaataaccg tcaggattga caccctccca attgtatgtt ttcatgcctc
393     3961 caaatcttgg aggctttttt atggttcgtt cttattaccc ttctgaatgt cacgctgatt
394     4021 attttgactt tgagcgtatc gaggctctta aacctgctat tgaggcttgt ggcatttcta
395     4081 ctctttctca atccccaatg cttggcttcc ataagcagat ggataaccgc atcaagctct
396     4141 tggaagagat tctgtctttt cgtatgcagg gcgttgagtt cgataatggt gatatgtatg
397     4201 ttgacggcca taaggctgct tctgacgttc gtgatgagtt tgtatctgtt actgagaagt
398     4261 taatggatga attggcacaa tgctacaatg tgctccccca acttgatatt aataacacta
399     4321 tagaccaccg ccccgaaggg gacgaaaaat ggtttttaga gaacgagaag acggttacgc
400     4381 agttttgccg caagctggct gctgaacgcc ctcttaagga tattcgcgat gagtataatt
401     4441 accccaaaaa gaaaggtatt aaggatgagt gttcaagatt gctggaggcc tccactatga
402     4501 aatcgcgtag aggctttgct attcagcgtt tgatgaatgc aatgcgacag gctcatgctg
403     4561 atggttggtt tatcgttttt gacactctca cgttggctga cgaccgatta gaggcgtttt
404     4621 atgataatcc caatgctttg cgtgactatt ttcgtgatat tggtcgtatg gttcttgctg
405     4681 ccgagggtcg caaggctaat gattcacacg ccgactgcta tcagtatttt tgtgtgcctg
406     4741 agtatggtac agctaatggc cgtcttcatt tccatgcggt gcactttatg cggacacttc
407     4801 ctacaggtag cgttgaccct aattttggtc gtcgggtacg caatcgccgc cagttaaata
408     4861 gcttgcaaaa tacgtggcct tatggttaca gtatgcccat cgcagttcgc tacacgcagg
409     4921 acgctttttc acgttctggt tggttgtggc ctgttgatgc taaaggtgag ccgcttaaag
410     4981 ctaccagtta tatggctgtt ggtttctatg tggctaaata cgttaacaaa aagtcagata
411     5041 tggaccttgc tgctaaaggt ctaggagcta aagaatggaa caactcacta aaaaccaagc
412     5101 tgtcgctact tcccaagaag ctgttcagaa tcagaatgag ccgcaacttc gggatgaaaa
413     5161 tgctcacaat gacaaatctg tccacggagt gcttaatcca acttaccaag ctgggttacg
414     5221 acgcgacgcc gttcaaccag atattgaagc agaacgcaaa aagagagatg agattgaggc
415     5281 tgggaaaagt tactgtagcc gacgttttgg cggcgcaacc tgtgacgaca aatctgctca
416     5341 aatttatgcg cgcttcgata aaaatgattg gcgtatccaa cctgca
417//
418