1@HD VN:1.5 SO:unknown 2@SQ SN:vir1 LN:800 3@RG ID:12345_1#2 4read_1 675 vir1 55 60 25S185M = 201 381 ACCCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACTCGGCTGCATGCTTAGTGCACTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTCTATCTTCTGCAGGCTGCTTACGGTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTT CC<CCFGGEGGGGGGGGEFDFCEFGGGGGGG<FFDGFGGGGGEDGG8FGGGFFGGGFGCFFFFGGGGGFGDFDCFCE<E9FFCGGG9FFFECFGFGGFCFGFFGGFGFGFGFCA<FCFFGFGGGEGFGC=DEGGGDCFE8BFGGGGDGGFFEEDF5AF8,FEFCFFCGFGGGCGCDGGGFCDGGGF9DFFE+++87:<DCFCB3,@,,3> MC:Z:210M AS:i:208 XS:i:0 MD:Z:1A208 NM:i:1 RG:Z:12345_1#2 5read_2 99 vir1 55 60 24S186M = 201 375 ACCCCCCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACTCGGCTGCATGCTTAGTGCACTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTCTATCTTCTGCAGGCTGCTTACGGTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTT 8888ACFGGGGFFFGGGGGGGFGGGGGGGGGFGGGGGGGGGDGGGGGGGGGGGGGGGGGGGGGGGGGGGGGEGGGGGGGAFGGGGGGGGGGGGGGGGGCFFGGGFDGFFGGGGGGGGGGGGGGGGGGFCFGGGGGGGGAFFGEGGFFGGGGGGGGGGGGGGGGGGGGGGGGGGGGFGGGGGGGGGFGGGEGEGCFGGGGGGDGGGFGGGG MC:Z:210M AS:i:205 XS:i:0 BC:Z:TGGGCCTA-ACTCCTAC QT:Z:CCCCCFGF CCCCCGGG MD:Z:205 NM:i:0 RG:Z:12345_1#2 6read_3 161 vir1 132 60 210M = 201 279 ATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTCTATCTTCTGCAGGCTGCTTACGGTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTCGTCCGGGTGTGACCGAAAGGTAAGATGGAGAGCCTTGTCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTTACAGGTTCGCGACG CCCCCGFGGGGGGFFGGGGGGGGGGGFGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGEGGGGGGGGFGGGFGGGDGGGG@FFEGGFGGEGGGGGGGGGGGGGGGGGFFGDFDGGGDGEG9F@FCFGFGGGGGGCFGGGGGCFAFBAFEGGGGFFFGFGFGGGGG<EGGGE MC:Z:210M AS:i:210 XS:i:0 MD:Z:210 NM:i:0 RG:Z:12345_1#2 7read_1 595 vir1 201 60 185M25S = 30 -381 TTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTCGTCCGGGTGTGACCGAAAGGTAAGATGGAGAGCCTTGTCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTTACAGGTTCGCGACGTGCTCGTACGTGGCTTTGGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAGATG F@=7DFFGFECC@F>:<BFFF;CFGGFBFCGF9E@EFC:7CCFFBFFGGFGGGGGGFF9?GGGGGGGGFGFF@CFGGGFB?<F8GFFGDGFF<9GFE,AFFFCEGFGGGGGGGGGGGGGGGFDFGGGGGGEEGGGGGGGGGGGGDGGGEGGGGGGGGGGFFFGGGGGGGGGF<CGGCGDGGGGGFFCGGGGGGGGFGGGGGGGFFCCCCC MC:Z:210M AS:i:210 XS:i:0 BC:Z:TGGGCCTA-ACTCCTAC QT:Z:9CCCCGGG CCCCCGG< MD:Z:210 NM:i:0 RG:Z:12345_1#2 8read_3 593 vir1 201 60 185M25S = 132 -279 TTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTCGTCCGGGTGTGACCGAAAGGTAAGATGGAGAGCCTTGTCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTTACAGGTTCGCGACGTGCTCGTACGTGGCTTTGGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAGATG GGGGGGGGGGGGEEGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGFGGGGGGGGGGGGGGGGGGGGGGGGGGFCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCCCCC MC:Z:210M AS:i:210 XS:i:0 BC:Z:TGGGCCTA-ACTCCTAC QT:Z:CCCCCGGG CCCCCGGG MD:Z:210 NM:i:0 RG:Z:12345_1#2 9read_2 659 vir1 201 60 185M25S = 36 -375 TTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTCGTCCGGGTGTGACCGAAAGGGAAGATGGAGAGCCTTGTCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTTACAGGTTCGCGACGTGCTCGTACGTGGCTTTGGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAGATG C*DGCEDCGFFFF7>:7GGECFCFGFCCDACGFCD:FC>EEGGGF:B39FBCF>GFF9GDC,GGFDGFGDFDGGGFFF7@7GFFGFFF?GDGFE9GFGGFFEGFCGGFDEDGFDGGGGGGFGFF9FGFGFGCF7@+7GGGGGGECEGFGGE8FEFFE9F9GGGCEFCGGFFGGGGFCGDGFEFDFEDF9GFDEGGGGCGGFCGDE@CCCC MC:Z:5S205M AS:i:205 XS:i:0 MD:Z:61T148 NM:i:1 RG:Z:12345_1#2 10read_4 609 vir1 407 60 86S124M = 517 320 CGAAATTACAGGTTCTCTCCTTGCTCTTCCTTTTCTTTTTCTCCTCCTTTTCTTCTTTCTTCTCCTCTTCCCTTCCCCCTCTTCCCGATGGCACTTGTGGCTTAGTAGAAGTTGAAAAAGGCGTTTTGCCTCAACTTGAACAGCCCTATGTGTTCATCAAACGTTCGGATGCTCGAACTGCACCTCATGGTCATGTTATGGTTGAGCTGG 86---;=;=6;;;;CCFEFGFE6;;=<CFGGFFEF-6,<;CCFGAFGEEEFEEFGGGEGFFFFGGFGEFFFFFEFFGGGGGGGGGGGGGGGGGGGGGGGGGFGGGGGGGGGGGGGGGGGGGGGEEEEGGGGGFGGGGGGGGGGGGGGGGGFGFFGGFGGGGGGFGGGGGGGGGGGGGGGGGGFFFGGGGGGGGGEDGGGGGGGGGGGGGG MC:Z:210M AS:i:124 XS:i:0 BC:Z:TGGGCCTA-ACTCCTAC QT:Z:CCCCCGGG CCCCCGGG MD:Z:124 NM:i:0 RG:Z:12345_1#2 11read_4 145 vir1 517 60 188M22S = 407 -320 TATGGTTGAGCTGGTAGCAGAACTCGAAGGCATTCAGTACGGTCGTAGTGGTGAGACACTTGGTGTCCTTGTCCCTCATGTGGGCGAAATACCAGTGGCTTACCGCAAGGTTCTTCTTCGTAAGAACGGTAATAAAGGAGCTGGTGGCCATAGTTACGGCGCCGATCTAAAGTCATTTGACTTAGGCGACGAGCTTGGCACTGATCCTTA GGE@D,GFGFGGGGGGGGCF@FCFB9CD@CGGGGGFCGGGGGGGGGGGGGFFBDCGGFFGFDGGFFBCGFE7GGGGGGGGGGGGGGGCGGGGGGGGFFDGF@FBFGGGGFEFEFFCGGFFEGCCE@GGGGGGGFDGCF@GGGGGGGGGGGGFEEGGGGGGGGGGGGGGGGGGGGGGGGGGG?GGGDGGGGGGGGGGGGFGGGGGGCCCCC MC:Z:86S124M AS:i:210 XS:i:0 MD:Z:210 NM:i:0 RG:Z:12345_1#2 12